MetaCyc Pathway: superpathway of L-lysine, L-threonine and L-methionine biosynthesis I in Pseudomonas fluorescens FW300-N2C3

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Reactions and Genes L-Phenylalanine (N)
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Aspartate transaminase (in reverse):
oxaloacetate + L-glutamate→L-aspartate + 2-oxoglutarate
(EC 2.6.1.1)
AO356_01275 +0.0
AO356_03090 -0.0
AO356_03985 -2.1
AO356_06565 +0.1
AO356_12070 -0.0
AO356_12595 -0.0
AO356_16875 -1.3
AO356_19495 -0.0
AO356_19865 -0.0
AO356_19905 -0.1
AO356_20345 +0.4
AO356_22880 +0.0
AO356_24005 +0.2
AO356_24115 +0.0
AO356_24720 -0.3
AO356_24975 +0.1
AO356_25355 -0.0
Aspartate kinase:
L-aspartate + ATP→L-aspartyl-4-phosphate + ADP
(EC 2.7.2.4)
AO356_18775 -1.0
Aspartate-semialdehyde dehydrogenase (in reverse):
L-aspartyl-4-phosphate + NADPH + H+→L-aspartate 4-semialdehyde + NADP+ + phosphate
(EC 1.2.1.11)
AO356_04970 -0.0
AO356_21420 N.D.
AO356_21425 +0.0
L-aspartate 4-semialdehyde + pyruvate→(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H+ + H2O (EC 4.3.3.7)
AO356_04530 N.D.
AO356_06755 -0.1
AO356_09275 +0.0
AO356_27160 -0.0
Homoserine dehydrogenase (in reverse):
L-aspartate 4-semialdehyde + NAD(P)H + H+→L-homoserine + NAD(P)+
(EC 1.1.1.3)
AO356_06375 N.D.
AO356_15140 -0.3
AO356_27140 +0.3
Homoserine kinase:
L-homoserine + ATP→O-phospho-L-homoserine + ADP + H+
(EC 2.7.1.39)
AO356_11475 N.D.
AO356_20835 +0.2
Homoserine O-succinyltransferase:
L-homoserine + succinyl-CoA→O-succinyl-L-homoserine + coenzyme A
(EC 2.3.1.46)
No genes
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NAD(P)H + H+→(S)-2,3,4,5-tetrahydrodipicolinate + NAD(P)+ + H2O (EC 1.17.1.8)
AO356_07490 N.D.
Cystathionine gamma-synthase:
O-succinyl-L-homoserine + L-cysteine→L-cystathionine + H+ + succinate
(EC 2.5.1.48)
AO356_15115 +0.2
AO356_21470 -2.8
2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase:
(S)-2,3,4,5-tetrahydrodipicolinate + succinyl-CoA + H2ON-succinyl-2-amino-6-ketopimelate + coenzyme A
(EC 2.3.1.117)
AO356_06070 N.D.
Threonine synthase:
O-phospho-L-homoserine + H2O→L-threonine + phosphate
(EC 4.2.3.1)
AO356_06370 N.D.
AO356_15130 -0.2
AO356_16855 -0.0
AO356_27150 +0.2
Cysteine-S-conjugate beta-lyase:
L-cystathionine + H2O→L-homocysteine + ammonium + pyruvate
(EC 4.4.1.13)
No genes
Succinyldiaminopimelate transaminase (in reverse):
N-succinyl-2-amino-6-ketopimelate + L-glutamateN-succinyl-L,L-2,6-diaminopimelate + 2-oxoglutarate
(EC 2.6.1.17)
AO356_06055 +0.3
AO356_18725 -0.6
5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase:
L-homocysteine + 5-methyltetrahydropteroyl tri-L-glutamate→L-methionine + tetrahydropteroyl tri-L-glutamate
(EC 2.1.1.14)
AO356_17740 +0.2
Methionine synthase:
L-homocysteine + a 5-methyltetrahydrofolate→L-methionine + a tetrahydrofolate
(EC 2.1.1.13)
AO356_00545 -0.1
Succinyl-diaminopimelate desuccinylase:
N-succinyl-L,L-2,6-diaminopimelate + H2O→L,L-diaminopimelate + succinate
(EC 3.5.1.18)
AO356_06090 N.D.
Diaminopimelate epimerase:
L,L-diaminopimelate→meso-diaminopimelate
(EC 5.1.1.7)
AO356_05160 -1.9
AO356_12735 -2.2
AO356_20725 -0.3
AO356_21240 -0.3
AO356_27850 +0.1
Diaminopimelate decarboxylase:
meso-diaminopimelate + H+→L-lysine + CO2
(EC 4.1.1.20)
AO356_12740 N.D.
AO356_28785 -0.1

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information