MetaCyc Pathway: L-asparagine biosynthesis III (tRNA-dependent) in Pseudomonas fluorescens FW300-N2E2

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Reactions and Genes antimonate 0.005 M
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Glutamate synthase (NADPH):
L-glutamine + H2O→ammonia + L-glutamate + H+ (EC 1.4.1.13; 1.4.1.14; 1.4.7.1; 2.6.1.85; 3.5.1.2; 3.5.1.38; 4.1.3.27; 4.3.2.10; 4.3.3.6; 6.3.4.2; 6.3.5.13; 6.3.5.2; 6.3.5.4; 6.3.5.5; 6.3.5.6; 6.3.5.7)
Pf6N2E2_1327 +0.1
Pf6N2E2_1717 +0.0
Pf6N2E2_1718 -0.2
Pf6N2E2_1828 -0.1
Pf6N2E2_2102 +0.4
Pf6N2E2_2224 -0.1
Pf6N2E2_2471 -0.2
Pf6N2E2_2542 -0.2
Pf6N2E2_2604 -0.1
Pf6N2E2_2840 -0.1
Pf6N2E2_2847 +0.1
Pf6N2E2_2929 -0.1
Pf6N2E2_3050 N.D.
Pf6N2E2_3151 -0.4
Pf6N2E2_3164 N.D.
Pf6N2E2_3288 N.D.
Pf6N2E2_3289 N.D.
Pf6N2E2_3290 N.D.
Pf6N2E2_3364 N.D.
Pf6N2E2_3365 N.D.
Pf6N2E2_3403 -0.0
Pf6N2E2_3707 N.D.
Pf6N2E2_3751 -0.0
Pf6N2E2_3752 +0.2
Pf6N2E2_4822 -0.0
Pf6N2E2_4824 +0.0
Pf6N2E2_4916 +0.6
Pf6N2E2_497 -0.2
Pf6N2E2_5461 +0.0
Pf6N2E2_5644 +0.3
Pf6N2E2_5756 +0.2
Pf6N2E2_6001 -1.4
Pf6N2E2_6056 -0.0
Aspartate--tRNA(Asn) ligase:
L-aspartate + tRNAAsn + ATP→an L-aspartyl-[tRNAAsn] + AMP + diphosphate (EC 6.1.1.23)
Pf6N2E2_5533 N.D.
Asparaginyl-tRNA synthase (glutamine-hydrolyzing):
an L-aspartyl-[tRNAAsn] + ATP→a 4-phosphooxy-L-aspartyl-[tRNAAsn] + ADP (EC 6.3.5.6)
Pf6N2E2_3288 N.D.
Pf6N2E2_3289 N.D.
Pf6N2E2_3290 N.D.
Pf6N2E2_3403 -0.0
Pf6N2E2_3707 N.D.
Asparaginyl-tRNA synthase (glutamine-hydrolyzing):
a 4-phosphooxy-L-aspartyl-[tRNAAsn] + ammonia→an L-asparaginyl-[tRNAAsn] + phosphate (EC 6.3.5.6)
Pf6N2E2_3288 N.D.
Pf6N2E2_3289 N.D.
Pf6N2E2_3290 N.D.
Pf6N2E2_3403 -0.0
Pf6N2E2_3707 N.D.

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information