MetaCyc Pathway: L-glutamate and L-glutamine biosynthesis in Pseudomonas fluorescens FW300-N2E2

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Reactions and Genes antimonate 0.005 M
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Glutamate dehydrogenase (NAD(P)(+)) (in reverse):
2-oxoglutarate + ammonium + NAD(P)H + H+→L-glutamate + NAD(P)+ + H2O (EC 1.4.1.3)
No genes
Glutamate synthase (ferredoxin) (in reverse):
2-oxoglutarate + L-glutamine + 2 H+ + 2 a reduced ferredoxin [iron-sulfur] cluster→2 L-glutamate + 2 an oxidized ferredoxin [iron-sulfur] cluster (EC 1.4.7.1)
Pf6N2E2_5461 +0.0
Glutamate synthase (NADH) (in reverse):
2-oxoglutarate + L-glutamine + NADH + H+→2 L-glutamate + NAD+ (EC 1.4.1.14)
Pf6N2E2_3751 -0.0
Pf6N2E2_497 -0.2
Glutamate synthase (NADPH):
L-glutamine + H2O→L-glutamate + ammonia + H+ (EC 1.4.1.13; 1.4.1.14; 1.4.7.1; 2.6.1.85; 3.5.1.2; 3.5.1.38; 4.1.3.27; 4.3.2.10; 4.3.3.6; 6.3.4.2; 6.3.5.13; 6.3.5.2; 6.3.5.4; 6.3.5.5; 6.3.5.6; 6.3.5.7)
Pf6N2E2_1327 +0.1
Pf6N2E2_1717 +0.0
Pf6N2E2_1718 -0.2
Pf6N2E2_1828 -0.1
Pf6N2E2_2102 +0.4
Pf6N2E2_2224 -0.1
Pf6N2E2_2471 -0.2
Pf6N2E2_2542 -0.2
Pf6N2E2_2604 -0.1
Pf6N2E2_2840 -0.1
Pf6N2E2_2847 +0.1
Pf6N2E2_2929 -0.1
Pf6N2E2_3050 N.D.
Pf6N2E2_3151 -0.4
Pf6N2E2_3164 N.D.
Pf6N2E2_3288 N.D.
Pf6N2E2_3289 N.D.
Pf6N2E2_3290 N.D.
Pf6N2E2_3364 N.D.
Pf6N2E2_3365 N.D.
Pf6N2E2_3403 -0.0
Pf6N2E2_3707 N.D.
Pf6N2E2_3751 -0.0
Pf6N2E2_3752 +0.2
Pf6N2E2_4822 -0.0
Pf6N2E2_4824 +0.0
Pf6N2E2_4916 +0.6
Pf6N2E2_497 -0.2
Pf6N2E2_5461 +0.0
Pf6N2E2_5644 +0.3
Pf6N2E2_5756 +0.2
Pf6N2E2_6001 -1.4
Pf6N2E2_6056 -0.0
Glutamate--ammonia ligase:
L-glutamate + ammonium + ATP→L-glutamine + ADP + H+ + phosphate (EC 6.3.1.2)
Pf6N2E2_2177 -0.4
Pf6N2E2_2730 -0.1
Pf6N2E2_3821 N.D.
Pf6N2E2_4509 +0.1
Pf6N2E2_4511 +0.2
Pf6N2E2_5686 -0.2
Glutamate dehydrogenase (NADP(+)) (in reverse):
2-oxoglutarate + ammonium + NADPH + H+→L-glutamate + NADP+ + H2O (EC 1.4.1.4)
Pf6N2E2_3751 -0.0
Glutamate synthase (NADPH) (in reverse):
2-oxoglutarate + ammonia + NADPH + 2 H+→L-glutamate + NADP+ + H2O (EC 1.4.1.13; 1.4.1.14)
Pf6N2E2_1327 +0.1
Pf6N2E2_1828 -0.1
Pf6N2E2_2224 -0.1
Pf6N2E2_2471 -0.2
Pf6N2E2_2604 -0.1
Pf6N2E2_2840 -0.1
Pf6N2E2_2847 +0.1
Pf6N2E2_2929 -0.1
Pf6N2E2_3151 -0.4
Pf6N2E2_3751 -0.0
Pf6N2E2_3752 +0.2
Pf6N2E2_4916 +0.6
Pf6N2E2_497 -0.2
Pf6N2E2_5756 +0.2

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information