MetaCyc Pathway: tetradecanoate biosynthesis (mitochondria) in Shewanella loihica PV-4

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Reactions and Genes Fusidic 0.0625 mg/ml
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3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
an acetoacetyl-[acp] + NADPH + H+→a (3R)-3-hydroxybutanoyl-[acp] + NADP+ (EC 1.1.1.100)
Shew_1322 -0.2
Shew_1326 +0.2
Shew_1407 +3.1
Shew_1603 N.D.
Shew_1673 (fabG) +1.0
Shew_3525 (fabG) +6.6
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxybutanoyl-[acp]→a (2E)-but-2-enoyl-[acp] + H2O (EC 4.2.1.59)
Shew_1817 N.D.
Shew_2624 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) (in reverse):
a (2E)-but-2-enoyl-[acp] + NADPH + H+→a butanoyl-[acp] + NADP+ (EC 1.3.1.10; 1.3.1.104; 1.3.1.39)
No genes
Beta-ketoacyl-acyl-carrier-protein synthase I:
a butanoyl-[acp] + malonyl-CoA + H+→a 3-oxohexanoyl-[acp] + CO2 + coenzyme A (EC 2.3.1.41)
Shew_2410 +8.6
Shew_3523 +6.5
Shew_3526 +6.6
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 3-oxohexanoyl-[acp] + NADPH + H+→a (3R)-3-hydroxyhexanoyl-[acp] + NADP+ (EC 1.1.1.100)
Shew_1322 -0.2
Shew_1326 +0.2
Shew_1407 +3.1
Shew_1603 N.D.
Shew_1673 (fabG) +1.0
Shew_3525 (fabG) +6.6
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxyhexanoyl-[acp]→a (2E)-hex-2-enoyl-[acp] + H2O (EC 4.2.1.59)
Shew_1817 N.D.
Shew_2624 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) (in reverse):
a (2E)-hex-2-enoyl-[acp] + NADPH + H+→a hexanoyl-[acp] + NADP+ (EC 1.3.1.10; 1.3.1.104; 1.3.1.39)
No genes
Beta-ketoacyl-acyl-carrier-protein synthase I:
a hexanoyl-[acp] + malonyl-CoA + H+→a 3-oxooctanoyl-[acp] + CO2 + coenzyme A (EC 2.3.1.41)
Shew_2410 +8.6
Shew_3523 +6.5
Shew_3526 +6.6
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 3-oxooctanoyl-[acp] + NADPH + H+→a (3R)-3-hydroxyoctanoyl-[acp] + NADP+ (EC 1.1.1.100)
Shew_1322 -0.2
Shew_1326 +0.2
Shew_1407 +3.1
Shew_1603 N.D.
Shew_1673 (fabG) +1.0
Shew_3525 (fabG) +6.6
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxyoctanoyl-[acp]→a (2E)-oct-2-enoyl-[acp] + H2O (EC 4.2.1.59)
Shew_1817 N.D.
Shew_2624 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) (in reverse):
a (2E)-oct-2-enoyl-[acp] + NADPH + H+→an octanoyl-[acp] + NADP+ (EC 1.3.1.10; 1.3.1.104; 1.3.1.39)
No genes
Beta-ketoacyl-acyl-carrier-protein synthase I:
an octanoyl-[acp] + malonyl-CoA + H+→a 3-oxodecanoyl-[acp] + CO2 + coenzyme A (EC 2.3.1.41)
Shew_2410 +8.6
Shew_3523 +6.5
Shew_3526 +6.6
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 3-oxodecanoyl-[acp] + NADPH + H+→a (3R)-3-hydroxydecanoyl-[acp] + NADP+ (EC 1.1.1.100)
Shew_1322 -0.2
Shew_1326 +0.2
Shew_1407 +3.1
Shew_1603 N.D.
Shew_1673 (fabG) +1.0
Shew_3525 (fabG) +6.6
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxydecanoyl-[acp]→a (2E)-dec-2-enoyl-[acp] + H2O (EC 4.2.1.59)
Shew_1817 N.D.
Shew_2624 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) (in reverse):
a (2E)-dec-2-enoyl-[acp] + NADPH + H+→a decanoyl-[acp] + NADP+ (EC 1.3.1.10; 1.3.1.104; 1.3.1.39)
No genes
Beta-ketoacyl-acyl-carrier-protein synthase I:
a decanoyl-[acp] + malonyl-CoA + H+→a 3-oxododecanoyl-[acp] + CO2 + coenzyme A (EC 2.3.1.41)
Shew_2410 +8.6
Shew_3523 +6.5
Shew_3526 +6.6
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 3-oxododecanoyl-[acp] + NADPH + H+→a (3R)-3-hydroxydodecanoyl-[acp] + NADP+ (EC 1.1.1.100)
Shew_1322 -0.2
Shew_1326 +0.2
Shew_1407 +3.1
Shew_1603 N.D.
Shew_1673 (fabG) +1.0
Shew_3525 (fabG) +6.6
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxydodecanoyl-[acp]→a (2E)-dodec-2-enoyl-[acp] + H2O (EC 4.2.1.59)
Shew_1817 N.D.
Shew_2624 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) (in reverse):
a (2E)-dodec-2-enoyl-[acp] + NADPH + H+→a dodecanoyl-[acp] + NADP+ (EC 1.3.1.10; 1.3.1.104; 1.3.1.39)
No genes
Dodecanoyl-[acyl-carrier-protein] hydrolase:
a dodecanoyl-[acp][nil] + H2O[nil]→laurate[nil] + a soluble [acyl-carrier protein][nil] + H+[nil] (EC 3.1.2.21)
No genes
Beta-ketoacyl-acyl-carrier-protein synthase I:
a dodecanoyl-[acp] + malonyl-CoA + H+→a 3-oxotetradecanoyl-[acp] + CO2 + coenzyme A (EC 2.3.1.41)
Shew_2410 +8.6
Shew_3523 +6.5
Shew_3526 +6.6
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 3-oxotetradecanoyl-[acp] + NADPH + H+→a (3R)-3-hydroxytetradecanoyl-[acp] + NADP+ (EC 1.1.1.100)
Shew_1322 -0.2
Shew_1326 +0.2
Shew_1407 +3.1
Shew_1603 N.D.
Shew_1673 (fabG) +1.0
Shew_3525 (fabG) +6.6
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxytetradecanoyl-[acp]→a (2E)-tetradec-2-enoyl-[acp] + H2O (EC 4.2.1.59)
Shew_1817 N.D.
Shew_2624 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) (in reverse):
a (2E)-tetradec-2-enoyl-[acp] + NADPH + H+→a myristoyl-[acp] + NADP+ (EC 1.3.1.10; 1.3.1.104; 1.3.1.39)
No genes
Oleoyl-[acyl-carrier-protein] hydrolase:
a myristoyl-[acp] + H2O→tetradecanoate + a soluble [acyl-carrier protein] + H+ (EC 3.1.2.14)
No genes

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information