MetaCyc Pathway: peptidoglycan biosynthesis IV (Enterococcus faecium) in Sphingomonas koreensis DSMZ 15582

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Reactions and Genes Fusidic 0.0005 mg/ml
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D-alanine--D-alanine ligase:
2 D-alanine + ATP→D-alanyl-D-alanine + ADP + H+ + phosphate (EC 6.3.2.4)
Ga0059261_3468 N.D.
Glutamate racemase:
L-glutamate→D-glutamate (EC 5.1.1.3)
Ga0059261_2446 N.D.
Ga0059261_2640 +0.2
UDP-N-acetylglucosamine 1-carboxyvinyltransferase:
UDP-N-acetyl-α-D-glucosamine + phosphoenolpyruvate→UDP-N-acetyl-α-D-glucosamine-enolpyruvate + phosphate (EC 2.5.1.7)
Ga0059261_1284 N.D.
UDP-N-acetyl-α-D-glucosamine-enolpyruvate + NADPH + H+→UDP-N-acetyl-α-D-muramate + NADP+ (EC 1.3.1.98)
Ga0059261_3466 N.D.
UDP-N-acetylmuramate--L-alanine ligase:
UDP-N-acetyl-α-D-muramate + L-alanine + ATP→UDP-N-acetyl-α-D-muramoyl-L-alanine + ADP + H+ + phosphate (EC 6.3.2.8)
Ga0059261_1415 -0.3
Ga0059261_3464 N.D.
UDP-N-acetylmuramoyl-L-alanine--D-glutamate ligase:
UDP-N-acetyl-α-D-muramoyl-L-alanine + D-glutamate + ATP→UDP-N-acetyl-α-D-muramoyl-L-alanyl-D-glutamate + ADP + H+ + phosphate (EC 6.3.2.9)
Ga0059261_3461 N.D.
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-lysine ligase:
UDP-N-acetyl-α-D-muramoyl-L-alanyl-D-glutamate + L-lysine + ATP→UDP-N-acetyl-α-D-muramoyl-L-alanyl-γ-D-glutamyl-L-lysine + ADP + H+ + phosphate (EC 6.3.2.7)
No genes
UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase:
UDP-N-acetyl-α-D-muramoyl-L-alanyl-γ-D-glutamyl-L-lysine + D-alanyl-D-alanine + ATP→UDP-N-acetyl-α-D-muramoyl-L-alanyl-γ-D-glutamyl-L-lysyl-D-alanyl-D-alanine + ADP + H+ + phosphate (EC 6.3.2.10)
Ga0059261_3459 N.D.
Phospho-N-acetylmuramoyl-pentapeptide-transferase:
UDP-N-acetyl-α-D-muramoyl-L-alanyl-γ-D-glutamyl-L-lysyl-D-alanyl-D-alanine + di-trans,octa-cis-undecaprenyl phosphate→Und-PP-MurNAc-L-Ala-γ-D-Glu-L-Lys-D-Ala-D-Ala + UMP (EC 2.7.8.13)
Ga0059261_3460 N.D.
acetylglucosaminyltransferase:
Und-PP-MurNAc-L-Ala-γ-D-Glu-L-Lys-D-Ala-D-Ala + UDP-N-acetyl-α-D-glucosamine→Und-PP-[β-D-GlcNAc-(1->4)]-MurNAc-L-Ala-γ-D-Glu-L-Lys-D-Ala-D-Ala + H+ + UDP (EC 2.4.1.227)
Ga0059261_3463 N.D.
Und-PP-[β-D-GlcNAc-(1->4)]-MurNAc-L-Ala-γ-D-Glu-L-Lys-D-Ala-D-Ala + ATP + L-glutamine + H2O→L-glutamate + Und-PP-[β-D-GlcNAc-(1->4)]-MurNAc-L-Ala-γ-D-iGln-L-Lys-D-Ala-D-Ala + ADP + H+ + phosphate (EC 6.3.5.13)
No genes
D-aspartate ligase:
Und-PP-[β-D-GlcNAc-(1->4)]-MurNAc-L-Ala-γ-D-iGln-L-Lys-D-Ala-D-Ala + ATP + D-aspartate→Und-PP-[β-D-GlcNAc-(1→4)]-MurNAc-L-Ala-γ-D-iGln-(6-N-β-D-Asp)-L-Lys-D-Ala-D-Ala + ADP + H+ + phosphate (EC 6.3.1.12)
No genes
Und-PP-[β-D-GlcNAc-(1→4)]-MurNAc-L-Ala-γ-D-iGln-(6-N-β-D-Asp)-L-Lys-D-Ala-D-Ala + ammonium + ATP→Und-PP-[β-D-GlcNAc-(1→4)]-MurNAc-L-Ala-γ-D-iGln-(6-N-β-D-Asn)-L-Lys-D-Ala-D-Ala + AMP + diphosphate + H+
No genes
Und-PP-[β-D-GlcNAc-(1→4)]-MurNAc-L-Ala-γ-D-iGln-(6-N-β-D-Asn)-L-Lys-D-Ala-D-Ala[in] + H+[out]→Und-PP-[β-D-GlcNAc-(1→4)]-MurNAc-L-Ala-γ-D-iGln-(6-N-β-D-Asn)-L-Lys-D-Ala-D-Ala[out] + H+[in]
No genes
Peptidoglycan glycosyltransferase:
2 Und-PP-[β-D-GlcNAc-(1→4)]-MurNAc-L-Ala-γ-D-iGln-(6-N-β-D-Asn)-L-Lys-D-Ala-D-Ala→a nascent peptidoglycan dimer (E. faeciums) + H+ + di-trans,octa-cis-undecaprenyl diphosphate (EC 2.4.1.129)
Ga0059261_1805 -0.4
Ga0059261_2181 -4.1
Ga0059261_2488 -0.0
Ga0059261_2697 -1.2
Ga0059261_3457 N.D.
Peptidoglycan glycosyltransferase:
Und-PP-[β-D-GlcNAc-(1→4)]-MurNAc-L-Ala-γ-D-iGln-(6-N-β-D-Asn)-L-Lys-D-Ala-D-Ala + a nascent peptidoglycan (E. faeciums)→a nascent peptidoglycan (E. faeciums) + H+ + di-trans,octa-cis-undecaprenyl diphosphate (EC 2.4.1.129)
Ga0059261_1805 -0.4
Ga0059261_2181 -4.1
Ga0059261_2488 -0.0
Ga0059261_2697 -1.2
Ga0059261_3457 N.D.
Serine-type D-Ala-D-Ala carboxypeptidase:
a nascent peptidoglycan (E. faeciums) + a peptidoglycan internal segment (E. faecium, tetrapeptide) + H+→D-alanine + a peptidoglycan with D,D cross-links (Enterococcus faecium) (EC 3.4.16.4)
Ga0059261_0122 N.D.
Ga0059261_1157 +0.0
Ga0059261_1299 -0.0

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information