MetaCyc Pathway: superpathway of L-methionine biosynthesis (transsulfuration) in Pseudomonas fluorescens FW300-N1B4

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Reactions and Genes L-Serine (N)
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Aspartate transaminase (in reverse):
oxaloacetate + L-glutamate→L-aspartate + 2-oxoglutarate (EC 2.6.1.1)
Pf1N1B4_160 -0.0
Pf1N1B4_2018 +0.0
Pf1N1B4_2139 +0.0
Pf1N1B4_261 +0.0
Pf1N1B4_3564 -0.1
Pf1N1B4_3771 -0.3
Pf1N1B4_386 -1.2
Pf1N1B4_4186 -0.1
Pf1N1B4_4425 -0.2
Pf1N1B4_4646 -0.1
Pf1N1B4_4672 N.D.
Pf1N1B4_4996 +0.0
Pf1N1B4_5070 +0.1
Pf1N1B4_510 -0.3
Pf1N1B4_5999 +0.2
Pf1N1B4_818 -0.0
Pf1N1B4_849 +0.1
Aspartate kinase:
L-aspartate + ATP→L-aspartyl-4-phosphate + ADP (EC 2.7.2.4)
Pf1N1B4_3451 -1.2
Aspartate-semialdehyde dehydrogenase (in reverse):
L-aspartyl-4-phosphate + NADPH + H+→L-aspartate 4-semialdehyde + NADP+ + phosphate (EC 1.2.1.11)
Pf1N1B4_10 -0.0
Pf1N1B4_11 N.D.
Pf1N1B4_3225 N.D.
Homoserine dehydrogenase (in reverse):
L-aspartate 4-semialdehyde + NAD(P)H + H+→L-homoserine + NAD(P)+ (EC 1.1.1.3)
Pf1N1B4_3013 N.D.
Homoserine O-succinyltransferase:
L-homoserine + succinyl-CoA→O-succinyl-L-homoserine + coenzyme A (EC 2.3.1.46)
No genes
Cystathionine gamma-synthase:
O-succinyl-L-homoserine + L-cysteine→L-cystathionine + H+ + succinate (EC 2.5.1.48)
No genes
Cysteine-S-conjugate beta-lyase:
L-cystathionine + H2O→L-homocysteine + ammonium + pyruvate (EC 4.4.1.13)
No genes
5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase:
L-homocysteine + 5-methyltetrahydropteroyl tri-L-glutamate→L-methionine + tetrahydropteroyl tri-L-glutamate (EC 2.1.1.14)
Pf1N1B4_783 +0.0
Methionine synthase:
L-homocysteine + a 5-methyltetrahydrofolate→L-methionine + a tetrahydrofolate (EC 2.1.1.13)
Pf1N1B4_5661 -0.0

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information