MetaCyc Pathway: superpathway of L-isoleucine biosynthesis I in Klebsiella michiganensis M5al

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Reactions and Genes D-Lactate (C)
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Aspartate transaminase (in reverse):
oxaloacetate + L-glutamate→L-aspartate + 2-oxoglutarate (EC 2.6.1.1)
BWI76_RS03690 -0.0
BWI76_RS06660 -0.0
BWI76_RS06750 +0.0
BWI76_RS07560 +0.1
BWI76_RS07570 +0.1
BWI76_RS08675 -0.1
BWI76_RS09870 -0.6
BWI76_RS10245 -0.1
BWI76_RS12985 +0.2
BWI76_RS13400 -0.0
BWI76_RS20205 -0.3
BWI76_RS22035 -0.2
BWI76_RS24235 -0.2
Aspartate kinase:
L-aspartate + ATP→L-aspartyl-4-phosphate + ADP (EC 2.7.2.4)
BWI76_RS00790 +0.6
BWI76_RS01790 +0.3
BWI76_RS04245 -4.0
Aspartate-semialdehyde dehydrogenase (in reverse):
L-aspartyl-4-phosphate + NADPH + H+→L-aspartate 4-semialdehyde + NADP+ + phosphate (EC 1.2.1.11)
BWI76_RS12880 -0.1
BWI76_RS20385 +0.2
BWI76_RS26220 N.D.
Homoserine dehydrogenase (in reverse):
L-aspartate 4-semialdehyde + NAD(P)H + H+→L-homoserine + NAD(P)+ (EC 1.1.1.3)
BWI76_RS00790 +0.6
BWI76_RS04245 -4.0
Homoserine kinase:
L-homoserine + ATP→O-phospho-L-homoserine + ADP + H+ (EC 2.7.1.39)
BWI76_RS04250 -5.1
Threonine synthase:
O-phospho-L-homoserine + H2O→L-threonine + phosphate (EC 4.2.3.1)
BWI76_RS04255 -5.3
Threonine ammonia-lyase:
L-threonine→(Z)-2-aminobutenoate + H2O (EC 4.3.1.19)
BWI76_RS00980 -3.5
1-aminocyclopropane-1-carboxylate deaminase:
(Z)-2-aminobutenoate→2-iminobutanoate (spontaneous) (EC 3.5.99.7; 4.3.1.19; 4.4.1.1; 4.4.1.11; 4.4.1.2)
BWI76_RS00980 -3.5
BWI76_RS16730 -0.1
BWI76_RS24210 +0.0
2-iminobutanoate + H2O→2-oxobutanoate + ammonium (spontaneous) (EC 3.5.99.10; 3.5.99.7; 4.3.1.19; 4.4.1.1; 4.4.1.11; 4.4.1.2)
BWI76_RS00980 -3.5
BWI76_RS02985 -0.7
BWI76_RS16730 -0.1
BWI76_RS24210 +0.0
Acetolactate synthase:
2-oxobutanoate + H+ + pyruvate→(S)-2-aceto-2-hydroxybutanoate + CO2 (EC 2.2.1.6)
BWI76_RS00960 -1.1
BWI76_RS00965 -2.0
BWI76_RS04695 +0.0
BWI76_RS04700 +0.1
BWI76_RS12900 -0.3
BWI76_RS16750 +0.2
BWI76_RS27810 -1.1
BWI76_RS27815 -2.7
Ketol-acid reductoisomerase (in reverse):
(S)-2-aceto-2-hydroxybutanoate + NADPH + H+→(R)-2,3-dihydroxy-3-methylpentanoate + NADP+ (EC 1.1.1.86)
BWI76_RS00990 -6.3
Dihydroxy-acid dehydratase:
(R)-2,3-dihydroxy-3-methylpentanoate→(3S)-3-methyl-2-oxopentanoate + H2O (EC 4.2.1.9)
BWI76_RS00975 -3.6
Branched-chain-amino-acid transaminase (in reverse):
(3S)-3-methyl-2-oxopentanoate + L-glutamate→L-isoleucine + 2-oxoglutarate (EC 2.6.1.42)
BWI76_RS00970 -0.7
BWI76_RS10365 +0.2
BWI76_RS24235 -0.2

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information