MetaCyc Pathway: L-citrulline biosynthesis in Pseudomonas fluorescens FW300-N1B4

Reactions and Genes	L-Arginine (N)
Arginase: L-arginine + H2O→L-ornithine + urea (EC 3.5.3.1)
No genes
Glutamate synthase (NADPH): L-glutamine + H2O→L-glutamate + ammonia + H+ (EC 1.4.1.13; 1.4.1.14; 1.4.7.1; 2.6.1.85; 3.5.1.2; 3.5.1.38; 4.1.3.27; 4.3.2.10; 4.3.3.6; 6.3.4.2; 6.3.5.13; 6.3.5.2; 6.3.5.4; 6.3.5.5; 6.3.5.6; 6.3.5.7)
Pf1N1B4_1100	N.D.
Pf1N1B4_1101	N.D.
Pf1N1B4_1102	N.D.
Pf1N1B4_1172	N.D.
Pf1N1B4_1173	N.D.
Pf1N1B4_1533	-1.4
Pf1N1B4_1534	-2.1
Pf1N1B4_2545	-3.4
Pf1N1B4_2547	-4.6
Pf1N1B4_2646	-0.1
Pf1N1B4_290	+0.7
Pf1N1B4_3102	N.D.
Pf1N1B4_3209	+0.1
Pf1N1B4_3229	+0.1
Pf1N1B4_3412	-0.3
Pf1N1B4_3592	+0.2
Pf1N1B4_3722	-0.4
Pf1N1B4_374	+0.2
Pf1N1B4_3797	-0.1
Pf1N1B4_3814	-0.2
Pf1N1B4_3940	N.D.
Pf1N1B4_4165	+0.0
Pf1N1B4_4436	-0.3
Pf1N1B4_4516	+0.2
Pf1N1B4_6037	+0.2
Pf1N1B4_706	N.D.
Pf1N1B4_839	N.D.
Pf1N1B4_875	+0.2
L-proline[in] + an electron-transfer quinone→(S)-1-pyrroline-5-carboxylate[in] + an electron-transfer quinol + H+[in] (EC 1.5.5.2)
Pf1N1B4_1489	-0.0
Glutamate 5-kinase: L-glutamate + ATP→γ-L-glutamyl 5-phosphate + ADP (EC 2.7.2.11)
Pf1N1B4_2800	N.D.
(S)-1-pyrroline-5-carboxylate + H+ + H2O→L-glutamate 5-semialdehyde (spontaneous)
No genes
Glutamate-5-semialdehyde dehydrogenase (in reverse): γ-L-glutamyl 5-phosphate + NADPH + H+→L-glutamate 5-semialdehyde + NADP+ + phosphate (EC 1.2.1.41)
Pf1N1B4_2688	+0.3
Pf1N1B4_4438	-0.0
Ornithine aminotransferase (in reverse): L-glutamate + L-glutamate 5-semialdehyde→L-ornithine + 2-oxoglutarate (EC 2.6.1.13)
Pf1N1B4_3440	-3.3
Ornithine carbamoyltransferase: L-ornithine + carbamoyl phosphate→L-citrulline + H+ + phosphate (EC 2.1.3.3)
Pf1N1B4_616	-0.3
Pf1N1B4_762	-0.5

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information