MetaCyc Pathway: L-isoleucine biosynthesis IV in Klebsiella michiganensis M5al

Reactions and Genes	Trisodium citrate (C)
Propionate--CoA ligase: propanoate + ATP + coenzyme A→propanoyl-CoA + AMP + diphosphate (EC 6.2.1.17)
BWI76_RS02095	-0.8
3-methyl-2-oxobutanoate dehydrogenase (ferredoxin) (in reverse): propanoyl-CoA + CO2 + H+ + 2 a reduced ferredoxin [iron-sulfur] cluster→2-oxobutanoate + coenzyme A + 2 an oxidized ferredoxin [iron-sulfur] cluster (EC 1.2.7.7)
No genes
Acetolactate synthase: 2-oxobutanoate + H+ + pyruvate→(S)-2-aceto-2-hydroxybutanoate + CO2 (EC 2.2.1.6)
BWI76_RS00960	-2.0
BWI76_RS00965	-4.7
BWI76_RS04695	-0.3
BWI76_RS04700	+0.3
BWI76_RS12900	-0.1
BWI76_RS16750	+0.0
BWI76_RS27810	-0.5
BWI76_RS27815	-1.4
(S)-2-aceto-2-hydroxybutanoate + NAD(P)H + H+→(R)-2,3-dihydroxy-3-methylpentanoate + NAD(P)+ (EC 1.1.1.383)
No genes
Dihydroxy-acid dehydratase: (R)-2,3-dihydroxy-3-methylpentanoate→(3S)-3-methyl-2-oxopentanoate + H2O (EC 4.2.1.9)
BWI76_RS00975	-3.8
Branched-chain-amino-acid transaminase (in reverse): (3S)-3-methyl-2-oxopentanoate + L-glutamate→L-isoleucine + 2-oxoglutarate (EC 2.6.1.42)
BWI76_RS00970	-1.5
BWI76_RS10365	-0.2
BWI76_RS24235	-0.4

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information