MetaCyc Pathway: dTMP de novo biosynthesis (mitochondrial) in Pseudomonas fluorescens FW300-N2E3

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Reactions and Genes outer cut, LB soft agar motility assay
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Dihydrofolate reductase (in reverse):
a 7,8-dihydrofolate + NADPH + H+→a tetrahydrofolate + NADP+ (EC 1.5.1.3)
AO353_04955 -0.2
AO353_08370 N.D.
AO353_28310 -0.6
Glycine hydroxymethyltransferase:
a tetrahydrofolate + L-serine→a 5,10-methylenetetrahydrofolate + glycine + H2O (EC 2.1.2.1)
AO353_07665 -0.1
AO353_10495 -1.8
AO353_10530 -0.0
AO353_14020 N.D.
AO353_14025 +2.6
AO353_29335 -0.2
Thymidylate synthase:
a 5,10-methylenetetrahydrofolate + dUMP→a 7,8-dihydrofolate + dTMP (EC 2.1.1.45)
AO353_08445 N.D.

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information