MetaCyc Pathway: glycine betaine degradation III in Pseudomonas fluorescens FW300-N2E3

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Reactions and Genes outer cut, LB soft agar motility assay
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glycine betaine + NADH + dioxygen + H+→N,N-dimethylglycine + formaldehyde + NAD+ + H2O (EC 1.14.13.251)
AO353_07675 -0.3
AO353_07680 -0.1
N,N-dimethylglycine + 2 an oxidized ferredoxin [iron-sulfur] cluster + H2O→sarcosine + formaldehyde + 2 H+ + 2 a reduced ferredoxin [iron-sulfur] cluster (EC 1.5.7.3)
AO353_07710 +0.2
sarcosine + 2 an oxidized ferredoxin [iron-sulfur] cluster + H2O→glycine + formaldehyde + 2 H+ + 2 a reduced ferredoxin [iron-sulfur] cluster (EC 1.5.7.3)
AO353_07710 +0.2
Glycine hydroxymethyltransferase (in reverse):
glycine + a 5,10-methylenetetrahydrofolate + H2O→L-serine + a tetrahydrofolate (EC 2.1.2.1)
AO353_07665 +0.6
AO353_10495 -2.0
AO353_10530 -0.4
AO353_14020 N.D.
AO353_14025 +2.6
AO353_29335 +0.3
L-serine ammonia-lyase:
L-serine→2-aminoprop-2-enoate + H2O (EC 4.3.1.17)
AO353_03505 -0.1
AO353_07775 +0.4
AO353_15500 -0.7
Tryptophanase:
2-aminoprop-2-enoate→2-iminopropanoate (spontaneous) (EC 4.1.99.1; 4.1.99.2; 4.3.1.13; 4.3.1.17; 4.3.1.18; 4.3.3.8; 4.4.1.1; 4.4.1.13; 4.4.1.15; 4.4.1.25; 4.4.1.28; 4.4.1.35; 4.5.1.2)
AO353_03505 -0.1
AO353_07775 +0.4
AO353_11680 -0.2
AO353_15500 -0.7
AO353_21740 -0.4
AO353_23295 +0.5
AO353_25210 -0.1
2-iminopropanoate + H2O→pyruvate + ammonium (spontaneous) (EC 3.5.99.10; 4.1.99.1; 4.1.99.2; 4.3.1.13; 4.3.1.17; 4.3.1.18; 4.3.3.8; 4.4.1.1; 4.4.1.13; 4.4.1.15; 4.4.1.25; 4.4.1.28; 4.4.1.35; 4.5.1.2)
AO353_03505 -0.1
AO353_07775 +0.4
AO353_09285 -0.3
AO353_11680 -0.2
AO353_15500 -0.7
AO353_21740 -0.4
AO353_23295 +0.5
AO353_24440 -0.5
AO353_25210 -0.1

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information