MetaCyc Pathway: lipid IV_A biosynthesis (generic) in Klebsiella michiganensis M5al

Reactions and Genes	a-Cyclodextrin (C)
Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase: UDP-N-acetyl-α-D-glucosamine + a (3R)-3-hydroxyacyl-[acyl-carrier protein]→UDP-3-O-[(3R)-3-hydroxyacyl]-N-acetyl-α-D-glucosamine + a soluble [acyl-carrier protein] (EC 2.3.1.129)
BWI76_RS05275	N.D.
UDP-3-O-[(3R)-3-hydroxyacyl]-N-acetyl-α-D-glucosamine + H2O→UDP-3-O-[(3R)-3-hydroxyacyl]-α-D-glucosamine + acetate (EC 3.5.1.108)
BWI76_RS04790	N.D.
UDP-3-O-[(3R)-3-hydroxyacyl]-α-D-glucosamine + a (3R)-3-hydroxyacyl-[acyl-carrier protein]→UDP-2-N,3-O-bis[(3R)-3-hydroxyacyl]-α-D-glucosamine + a soluble [acyl-carrier protein] + H+ (EC 2.3.1.191)
BWI76_RS03260	-2.5
BWI76_RS05265	N.D.
UDP-2-N,3-O-bis[(3R)-3-hydroxyacyl]-α-D-glucosamine + H2O→a lipid X + 2 H+ + UMP (EC 3.6.1.54)
BWI76_RS07080	N.D.
Lipid-A-disaccharide synthase: a lipid X + UDP-2-N,3-O-bis[(3R)-3-hydroxyacyl]-α-D-glucosamine→a lipid A disaccharide + H+ + UDP (EC 2.4.1.182)
BWI76_RS05280	N.D.
Tetraacyldisaccharide 4'-kinase: a lipid A disaccharide + ATP→a lipid IVA + ADP + H+ (EC 2.7.1.130)
BWI76_RS09800	N.D.

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information