MetaCyc Pathway: L-arginine biosynthesis II (acetyl cycle) in Pseudomonas fluorescens FW300-N1B4

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Glutamate--ammonia ligase:
L-glutamate + ammonium + ATP→L-glutamine + ADP + H+ + phosphate (EC 6.3.1.2)
Pf1N1B4_1606 N.D.
Pf1N1B4_2254 +0.2
Pf1N1B4_2256 -0.3
Pf1N1B4_3342 -0.0
Pf1N1B4_3464 +0.1
Pf1N1B4_4245 +0.2
Pf1N1B4_4354 +0.5
Pf1N1B4_4826 +1.1
Pf1N1B4_5853 -0.5
Amino-acid N-acetyltransferase:
L-glutamate + acetyl-CoA→N-acetyl-L-glutamate + coenzyme A + H+ (EC 2.3.1.1)
Pf1N1B4_2016 +0.0
Pf1N1B4_2243 N.D.
Pf1N1B4_656 +2.6
Acetylglutamate kinase:
N-acetyl-L-glutamate + ATP→N-acetylglutamyl-phosphate + ADP (EC 2.7.2.8)
Pf1N1B4_2121 N.D.
Carbamoyl-phosphate synthase (glutamine-hydrolyzing):
L-glutamine + 2 ATP + hydrogen carbonate + H2O→L-glutamate + carbamoyl phosphate + 2 ADP + 2 H+ + phosphate (EC 6.3.5.5)
Pf1N1B4_1172 N.D.
Pf1N1B4_1173 N.D.
N-acetyl-gamma-glutamyl-phosphate reductase (in reverse):
N-acetylglutamyl-phosphate + NADPH + H+→N-acetyl-L-glutamate 5-semialdehyde + NADP+ + phosphate (EC 1.2.1.38)
Pf1N1B4_2559 N.D.
Ornithine carbamoyltransferase:
carbamoyl phosphate + L-ornithine→L-citrulline + H+ + phosphate (EC 2.1.3.3)
Pf1N1B4_616 -0.6
Pf1N1B4_762 -2.7
Acetylornithine transaminase (in reverse):
N-acetyl-L-glutamate 5-semialdehyde + L-glutamate→N-acetyl-L-ornithine + 2-oxoglutarate (EC 2.6.1.11)
Pf1N1B4_2980 -0.5
Pf1N1B4_3440 +1.5
Pf1N1B4_4910 +0.0
Argininosuccinate synthase:
L-citrulline + ATP + L-aspartate→L-arginino-succinate + AMP + diphosphate + H+ (EC 6.3.4.5)
Pf1N1B4_742 -3.8
Argininosuccinate lyase:
L-arginino-succinate→L-arginine + fumarate (EC 4.3.2.1)
Pf1N1B4_2183 N.D.
Pf1N1B4_4806 -1.3
Glutamate N-acetyltransferase:
L-glutamate + N-acetyl-L-ornithine→N-acetyl-L-glutamate + L-ornithine (EC 2.3.1.35)
Pf1N1B4_656 +2.6

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information