MetaCyc Pathway: rosmarinic acid biosynthesis I in Pseudomonas fluorescens FW300-N2E3

Add experiment(s):


Reactions and Genes L-tyrosine (N)
remove
Phenylalanine ammonia-lyase:
L-phenylalanine→trans-cinnamate + ammonium (EC 4.3.1.24; 4.3.1.25; 5.4.3.10)
No genes
Aspartate transaminase:
L-tyrosine + 2-oxoglutarate→3-(4-hydroxyphenyl)pyruvate + L-glutamate (EC 2.6.1.1; 2.6.1.27; 2.6.1.5; 2.6.1.57)
AO353_01120 -0.1
AO353_01210 +0.3
AO353_01655 +0.0
AO353_02065 +0.0
AO353_02295 -0.1
AO353_05105 -1.0
AO353_09180 -0.1
AO353_09990 +0.0
AO353_15915 +0.0
AO353_17700 -0.1
AO353_18575 -2.6
AO353_19150 -0.1
AO353_20540 +0.2
AO353_20995 +0.1
AO353_22840 -0.1
AO353_22920 -0.3
AO353_23080 +0.1
AO353_24850 -0.1
AO353_26890 +0.0
AO353_28150 +0.3
AO353_29325 +0.2
Hydroxyphenylpyruvate reductase (in reverse):
3-(4-hydroxyphenyl)pyruvate + NAD(P)H + H+→(R)-3-(4-hydroxyphenyl)lactate + NAD(P)+ (EC 1.1.1.237)
No genes
trans-cinnamate + dioxygen + a reduced [NADPH-hemoprotein reductase]→4-coumarate + an oxidized [NADPH-hemoprotein reductase] + H2O (EC 1.14.14.91)
No genes
4-coumarate--CoA ligase:
4-coumarate + ATP + coenzyme A→(E)-4-coumaroyl-CoA + AMP + diphosphate (EC 6.2.1.12)
No genes
(R)-3-(4-hydroxyphenyl)lactate + (E)-4-coumaroyl-CoA→4-coumaroyl-4'-hydroxyphenyllactate + coenzyme A
No genes
4-coumaroyl-4'-hydroxyphenyllactate + dioxygen + a reduced [NADPH-hemoprotein reductase]→caffeoyl-4'-hydroxyphenyllactate + an oxidized [NADPH-hemoprotein reductase] + H2O
No genes
4-coumaroyl-4'-hydroxyphenyllactate + dioxygen + a reduced [NADPH-hemoprotein reductase]→4-coumaroyl-3',4'-dihydroxyphenyllactate + an oxidized [NADPH-hemoprotein reductase] + H2O
No genes
caffeoyl-4'-hydroxyphenyllactate + dioxygen + a reduced [NADPH-hemoprotein reductase]→(R)-rosmarinate + an oxidized [NADPH-hemoprotein reductase] + H2O
No genes
4-coumaroyl-3',4'-dihydroxyphenyllactate + dioxygen + a reduced [NADPH-hemoprotein reductase]→(R)-rosmarinate + an oxidized [NADPH-hemoprotein reductase] + H2O
No genes

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information