MetaCyc Pathway: 2-methyl-branched fatty acid β-oxidation in Pseudomonas fluorescens SBW25-INTG

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Reactions and Genes NAG (C)
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Butyrate--CoA ligase:
a short-chain 2-methyl-branched fatty acid + ATP + coenzyme A→a short-chain 2-methyl fatty acyl-CoA + AMP + diphosphate (EC 6.2.1.2)
No genes
a long-chain 2-methyl fatty acyl-CoA + an oxidized electron-transfer flavoprotein + H+→a long-chain (2E)-2-methyl-2-enoyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.8)
PFLU_RS04175 +0.5
Butyrate--CoA ligase:
a medium-chain 2-methyl-branched fatty acid + ATP + coenzyme A→a medium-chain 2-methyl fatty acyl-CoA + AMP + diphosphate (EC 6.2.1.2)
No genes
a short-chain 2-methyl fatty acyl-CoA + an oxidized electron-transfer flavoprotein + H+→a short-chain (2E)-2-methyl-2-enoyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.5)
No genes
a medium-chain 2-methyl fatty acyl-CoA + an oxidized electron-transfer flavoprotein + H+→a medium-chain (2E)-2-methyl-2-enoyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.7)
PFLU_RS02225 +0.4
PFLU_RS04175 +0.5
PFLU_RS16060 +0.4
PFLU_RS26520 +0.2
PFLU_RS27605 -2.0
PFLU_RS27610 -0.0
PFLU_RS27620 -5.0
Enoyl-CoA hydratase (in reverse):
a long-chain (2E)-2-methyl-2-enoyl-CoA + H2O→a long-chain (3S)-3-hydroxy-2-methylacyl-CoA (EC 4.2.1.17)
PFLU_RS07105 -0.0
PFLU_RS07650 -5.1
PFLU_RS08485 -1.5
PFLU_RS10835 +0.5
PFLU_RS12025 +0.5
PFLU_RS13550 -0.2
PFLU_RS14775 -0.2
PFLU_RS14785 -0.0
PFLU_RS16080 +0.1
PFLU_RS22820 -0.3
PFLU_RS22840 +0.1
PFLU_RS24260 -0.0
Enoyl-CoA hydratase (in reverse):
a short-chain (2E)-2-methyl-2-enoyl-CoA + H2O→a short-chain (3S)-3-hydroxy-2-methylacyl-CoA (EC 4.2.1.17)
PFLU_RS07105 -0.0
PFLU_RS07650 -5.1
PFLU_RS08485 -1.5
PFLU_RS10835 +0.5
PFLU_RS12025 +0.5
PFLU_RS13550 -0.2
PFLU_RS14775 -0.2
PFLU_RS14785 -0.0
PFLU_RS16080 +0.1
PFLU_RS22820 -0.3
PFLU_RS22840 +0.1
PFLU_RS24260 -0.0
Enoyl-CoA hydratase (in reverse):
a medium-chain (2E)-2-methyl-2-enoyl-CoA + H2O→a medium-chain (3S)-3-hydroxy-2-methylacyl-CoA (EC 4.2.1.17)
PFLU_RS07105 -0.0
PFLU_RS07650 -5.1
PFLU_RS08485 -1.5
PFLU_RS10835 +0.5
PFLU_RS12025 +0.5
PFLU_RS13550 -0.2
PFLU_RS14775 -0.2
PFLU_RS14785 -0.0
PFLU_RS16080 +0.1
PFLU_RS22820 -0.3
PFLU_RS22840 +0.1
PFLU_RS24260 -0.0
3-hydroxyacyl-CoA dehydrogenase:
a long-chain (3S)-3-hydroxy-2-methylacyl-CoA + NAD+→a long-chain 2-methyl-3-oxoacyl-CoA + NADH + H+ (EC 1.1.1.35)
PFLU_RS07650 -5.1
PFLU_RS11090 +0.2
PFLU_RS22840 +0.1
3-hydroxy-2-methylbutyryl-CoA dehydrogenase:
a short-chain (3S)-3-hydroxy-2-methylacyl-CoA + NAD+→a short-chain 2-methyl-3-oxoacyl-CoA + NADH + H+ (EC 1.1.1.178)
PFLU_RS11090 +0.2
3-hydroxyacyl-CoA dehydrogenase:
a medium-chain (3S)-3-hydroxy-2-methylacyl-CoA + NAD+→a medium-chain 2-methyl-3-oxoacyl-CoA + NADH + H+ (EC 1.1.1.35)
PFLU_RS07650 -5.1
PFLU_RS11090 +0.2
PFLU_RS22840 +0.1
Acetyl-CoA C-acyltransferase (in reverse):
a long-chain 2-methyl-3-oxoacyl-CoA + coenzyme A→a long-chain 2-methyl fatty acyl-CoA + propanoyl-CoA (EC 2.3.1.16)
PFLU_RS03225 +0.1
PFLU_RS06735 -0.6
PFLU_RS07655 -4.5
PFLU_RS10575 +0.2
PFLU_RS14765 -0.3
PFLU_RS16065 -0.4
PFLU_RS17190 -0.2
PFLU_RS21180 -0.4
PFLU_RS22845 +0.3
Acetyl-CoA C-acyltransferase (in reverse):
a short-chain 2-methyl-3-oxoacyl-CoA + coenzyme A→a short-chain 2-methyl fatty acyl-CoA + propanoyl-CoA (EC 2.3.1.16)
PFLU_RS03225 +0.1
PFLU_RS06735 -0.6
PFLU_RS07655 -4.5
PFLU_RS10575 +0.2
PFLU_RS14765 -0.3
PFLU_RS16065 -0.4
PFLU_RS17190 -0.2
PFLU_RS21180 -0.4
PFLU_RS22845 +0.3
Acetyl-CoA C-acyltransferase (in reverse):
a medium-chain 2-methyl-3-oxoacyl-CoA + coenzyme A→a medium-chain 2-methyl fatty acyl-CoA + propanoyl-CoA (EC 2.3.1.16)
PFLU_RS03225 +0.1
PFLU_RS06735 -0.6
PFLU_RS07655 -4.5
PFLU_RS10575 +0.2
PFLU_RS14765 -0.3
PFLU_RS16065 -0.4
PFLU_RS17190 -0.2
PFLU_RS21180 -0.4
PFLU_RS22845 +0.3

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information