MetaCyc Pathway: L-citrulline biosynthesis in Pseudomonas fluorescens FW300-N2E3

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Reactions and Genes L-Phenylalanine (N)
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Arginase:
L-arginine + H2O→L-ornithine + urea (EC 3.5.3.1)
No genes
Glutamate synthase (NADPH):
L-glutamine + H2O→L-glutamate + ammonia + H+ (EC 1.4.1.13; 1.4.1.14; 1.4.7.1; 2.6.1.85; 3.5.1.2; 3.5.1.38; 4.1.3.27; 4.3.2.10; 4.3.3.6; 6.3.4.2; 6.3.5.13; 6.3.5.2; 6.3.5.4; 6.3.5.5; 6.3.5.6; 6.3.5.7)
AO353_00925 +0.2
AO353_01150 -0.0
AO353_02570 +0.5
AO353_03130 +0.1
AO353_03905 -0.0
AO353_04395 N.D.
AO353_05255 N.D.
AO353_05260 N.D.
AO353_05265 N.D.
AO353_05630 N.D.
AO353_05635 N.D.
AO353_06730 -0.0
AO353_07220 -4.7
AO353_07230 -3.9
AO353_10550 -0.2
AO353_12515 -1.2
AO353_12520 -1.4
AO353_15955 N.D.
AO353_16160 +0.1
AO353_16550 N.D.
AO353_17090 +0.0
AO353_17175 -0.7
AO353_19025 +0.2
AO353_20130 -0.0
AO353_20805 -0.3
AO353_26575 +0.2
AO353_26790 -0.2
L-proline[in] + an electron-transfer quinone→(S)-1-pyrroline-5-carboxylate[in] + an electron-transfer quinol + H+[in] (EC 1.5.5.2)
AO353_12810 +2.0
Glutamate 5-kinase:
L-glutamate + ATP→γ-L-glutamyl 5-phosphate + ADP (EC 2.7.2.11)
AO353_14115 -1.9
(S)-1-pyrroline-5-carboxylate + H+ + H2O→L-glutamate 5-semialdehyde (spontaneous)
No genes
Glutamate-5-semialdehyde dehydrogenase (in reverse):
γ-L-glutamyl 5-phosphate + NADPH + H+→L-glutamate 5-semialdehyde + NADP+ + phosphate (EC 1.2.1.41)
AO353_06495 -3.4
Ornithine aminotransferase (in reverse):
L-glutamate + L-glutamate 5-semialdehyde→L-ornithine + 2-oxoglutarate (EC 2.6.1.13)
AO353_03025 -0.5
AO353_07470 +0.1
Ornithine carbamoyltransferase:
L-ornithine + carbamoyl phosphate→L-citrulline + H+ + phosphate (EC 2.1.3.3)
AO353_03475 -0.1
AO353_04155 -2.9

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information