MetaCyc Pathway: crotonate fermentation (to acetate and cyclohexane carboxylate) in Pseudomonas fluorescens FW300-N2E3

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Reactions and Genes butyrate (C)
remove
acetyl-CoA + crotonate→acetate + crotonyl-CoA
No genes
crotonyl-CoA + CO2→(E)-glutaconyl-CoA + H+ (EC 1.3.8.6)
No genes
crotonyl-CoA + H2O→(S)-3-hydroxybutanoyl-CoA (EC 4.2.1.150)
AO353_00510 -1.5
AO353_03780 -0.0
AO353_17555 -0.1
AO353_19125 N.D.
AO353_23055 +0.1
AO353_24830 -0.5
(E)-glutaconyl-CoA + a reduced electron-transfer flavoprotein→glutaryl-CoA + an oxidized electron-transfer flavoprotein + H+ (EC 1.3.8.6)
No genes
3-hydroxyacyl-CoA dehydrogenase:
(S)-3-hydroxybutanoyl-CoA + NAD+→acetoacetyl-CoA + NADH + H+ (EC 1.1.1.35)
AO353_00510 -1.5
AO353_01155 +0.0
AO353_27535 -3.3
Acetyl-CoA C-acyltransferase (in reverse):
acetoacetyl-CoA + coenzyme A→2 acetyl-CoA (EC 2.3.1.16; 2.3.1.9)
AO353_00505 -0.5
AO353_01065 +3.0
AO353_01160 +0.1
AO353_05785 +0.0
AO353_13880 +0.1
AO353_17205 -1.2
AO353_25685 -2.7
AO353_27530 -2.4
AO353_27945 -0.2
acetyl-CoA + glutaryl-CoA→3-oxopimeloyl-CoA + coenzyme A
No genes
3-hydroxypimeloyl-CoA dehydrogenase (in reverse):
3-oxopimeloyl-CoA + NADH + H+→3-hydroxypimeloyl-CoA + NAD+ (EC 1.1.1.259)
No genes
Acetate--CoA ligase (ADP-forming) (in reverse):
acetyl-CoA + ADP + phosphate→acetate + ATP + coenzyme A (EC 6.2.1.13)
No genes
3-hydroxypimeloyl-CoA + H+→2-hydroxy-6-oxocyclohexane-1-carbonyl-CoA + H2O (EC 3.7.1.21)
No genes
2-hydroxy-6-oxocyclohexane-1-carbonyl-CoA→6-oxocyclohex-1-ene-1-carbonyl-CoA + H2O (EC 3.7.1.21)
No genes
6-oxocyclohex-1-ene-1-carbonyl-CoA + NADH + H+→6-hydroxycyclohex-1-ene-1-carbonyl-CoA + NAD+ (EC 1.1.1.368)
No genes
Cyclohexa-1,5-dienecarbonyl-CoA hydratase:
6-hydroxycyclohex-1-ene-1-carbonyl-CoA→cyclohex-1,5-diene-1-carbonyl-CoA + H2O (EC 4.2.1.100)
No genes
cyclohex-1,5-diene-1-carbonyl-CoA + a reduced electron-transfer flavoprotein→cyclohex-1-ene-1-carbonyl-CoA + an oxidized electron-transfer flavoprotein + H+ (EC 1.3.8.10)
No genes
cyclohex-1-ene-1-carbonyl-CoA + a reduced electron-transfer flavoprotein→cyclohexane-1-carbonyl-CoA + an oxidized electron-transfer flavoprotein + H+ (EC 1.3.8.11)
No genes
cyclohexane-1-carbonyl-CoA + H2O→cyclohexane-1-carboxylate + coenzyme A + H+
No genes

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information