MetaCyc Pathway: glycerol degradation to butanol in Pseudomonas fluorescens FW300-N2E3

Reactions and Genes	butyrate (C)
Glycerol dehydrogenase: glycerol + NAD+→dihydroxyacetone + NADH + H+ (EC 1.1.1.6)
No genes
Glycerone kinase: dihydroxyacetone + ATP→glycerone phosphate + ADP + H+ (EC 2.7.1.29)
No genes
Triose-phosphate isomerase (in reverse): glycerone phosphate→D-glyceraldehyde 3-phosphate (EC 5.3.1.1)
AO353_05590	N.D.
AO353_21370	+0.0
Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating): D-glyceraldehyde 3-phosphate + NAD+ + phosphate→3-phospho-D-glyceroyl phosphate + NADH + H+ (EC 1.2.1.12)
AO353_00430	-4.2
AO353_03425	+0.1
Phosphoglycerate kinase (in reverse): 3-phospho-D-glyceroyl phosphate + ADP→3-phospho-D-glycerate + ATP (EC 2.7.2.3)
AO353_07945	N.D.
3-phospho-D-glycerate→2-phospho-D-glycerate (EC 5.4.2.12)
AO353_12115	-3.8
Phosphopyruvate hydratase: 2-phospho-D-glycerate→phosphoenolpyruvate + H2O (EC 4.2.1.11)
AO353_16560	N.D.
Pyruvate kinase (in reverse): phosphoenolpyruvate + ADP + H+→pyruvate + ATP (EC 2.7.1.40)
AO353_01940	-0.1
AO353_03775	-0.2
Pyruvate synthase: pyruvate + coenzyme A + 2 an oxidized ferredoxin [iron-sulfur] cluster→acetyl-CoA + CO2 + H+ + 2 a reduced ferredoxin [iron-sulfur] cluster (EC 1.2.7.1)
No genes
Acetyl-CoA C-acyltransferase: 2 acetyl-CoA→acetoacetyl-CoA + coenzyme A (EC 2.3.1.16; 2.3.1.9)
AO353_00505	-0.5
AO353_01065	+3.0
AO353_01160	+0.1
AO353_05785	+0.0
AO353_13880	+0.1
AO353_17205	-1.2
AO353_25685	-2.7
AO353_27530	-2.4
AO353_27945	-0.2
3-hydroxyacyl-CoA dehydrogenase (in reverse): acetoacetyl-CoA + NADH + H+→(S)-3-hydroxybutanoyl-CoA + NAD+ (EC 1.1.1.35)
AO353_00510	-1.5
AO353_01155	+0.0
AO353_27535	-3.3
(S)-3-hydroxybutanoyl-CoA→crotonyl-CoA + H2O (EC 4.2.1.150)
AO353_00510	-1.5
AO353_03780	-0.0
AO353_17555	-0.1
AO353_19125	N.D.
AO353_23055	+0.1
AO353_24830	-0.5
crotonyl-CoA[cytosol] + 2 NADH[cytosol] + 2 an oxidized ferredoxin [iron-sulfur] cluster[cytosol]→butanoyl-CoA[cytosol] + 2 NAD+[cytosol] + 2 a reduced ferredoxin [iron-sulfur] cluster[cytosol] (EC 1.3.1.109)
No genes
Butanal dehydrogenase (in reverse): butanoyl-CoA + NAD(P)H + H+→1-butanal + coenzyme A + NAD(P)+ (EC 1.2.1.57)
No genes
Alcohol dehydrogenase (NADP(+)) (in reverse): 1-butanal + NADPH + H+→butan-1-ol + NADP+ (EC 1.1.1.2)
AO353_22915	-0.0
1-butanal + NADH + H+→butan-1-ol + NAD+
No genes

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information