MetaCyc Pathway: pyruvate fermentation to butanol II (engineered) in Pseudomonas fluorescens FW300-N2E3

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Reactions and Genes butyrate (C)
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Acetyl-CoA C-acyltransferase:
2 acetyl-CoA→acetoacetyl-CoA + coenzyme A (EC 2.3.1.16; 2.3.1.9)
AO353_00505 -0.5
AO353_01065 +3.0
AO353_01160 +0.1
AO353_05785 +0.0
AO353_13880 +0.1
AO353_17205 -1.2
AO353_25685 -2.7
AO353_27530 -2.4
AO353_27945 -0.2
3-hydroxyacyl-CoA dehydrogenase (in reverse):
acetoacetyl-CoA + NADH + H+→(S)-3-hydroxybutanoyl-CoA + NAD+ (EC 1.1.1.35)
AO353_00510 -1.5
AO353_01155 +0.0
AO353_27535 -3.3
(S)-3-hydroxybutanoyl-CoA→crotonyl-CoA + H2O (EC 4.2.1.150)
AO353_00510 -1.5
AO353_03780 -0.0
AO353_17555 -0.1
AO353_19125 N.D.
AO353_23055 +0.1
AO353_24830 -0.5
Trans-2-enoyl-CoA reductase (NAD(+)) (in reverse):
crotonyl-CoA + NADH + H+→butanoyl-CoA + NAD+ (EC 1.3.1.44)
AO353_00670 +0.6
AO353_01500 +0.2
AO353_15135 -0.0
AO353_15225 +0.2
AO353_24835 -0.1
AO353_25670 +0.0
AO353_25680 -2.6
AO353_28600 -0.1
Butanal dehydrogenase (in reverse):
butanoyl-CoA + NAD(P)H + H+→1-butanal + coenzyme A + NAD(P)+ (EC 1.2.1.57)
No genes
1-butanal + NADH + H+→butan-1-ol + NAD+
No genes

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information