MetaCyc Pathway: superpathway of L-methionine biosynthesis (by sulfhydrylation) in Pseudomonas fluorescens FW300-N2E3

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Reactions and Genes L-Phenylalanine (C)
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Aspartate transaminase (in reverse):
oxaloacetate + L-glutamate→L-aspartate + 2-oxoglutarate (EC 2.6.1.1)
AO353_01120 +0.0
AO353_01210 -0.3
AO353_01655 -0.1
AO353_02295 +0.2
AO353_09180 +0.1
AO353_09990 +0.1
AO353_15915 +0.1
AO353_17700 +0.1
AO353_18575 -3.2
AO353_19150 -0.1
AO353_20995 -0.1
AO353_22840 -0.0
AO353_22920 -0.4
AO353_23080 +0.0
AO353_24850 -0.1
AO353_26890 +0.0
AO353_28150 +0.3
AO353_29325 -0.2
Sulfate adenylyltransferase:
sulfate + ATP + H+→adenosine 5'-phosphosulfate + diphosphate (EC 2.7.7.4)
AO353_05075 -0.6
AO353_05080 -0.6
AO353_12965 -0.2
Adenylyl-sulfate kinase:
adenosine 5'-phosphosulfate + ATP→3'-phosphoadenylyl-sulfate + ADP + H+ (EC 2.7.1.25)
AO353_05075 -0.6
AO353_11305 +0.1
AO353_12965 -0.2
Aspartate kinase:
L-aspartate + ATP→L-aspartyl-4-phosphate + ADP (EC 2.7.2.4)
AO353_02975 -0.3
Phosphoadenylyl-sulfate reductase (thioredoxin) (in reverse):
3'-phosphoadenylyl-sulfate + a reduced thioredoxin→sulfite + adenosine 3',5'-bisphosphate + an oxidized thioredoxin + 2 H+ (EC 1.8.4.8)
AO353_00935 N.D.
Aspartate-semialdehyde dehydrogenase (in reverse):
L-aspartyl-4-phosphate + NADPH + H+→L-aspartate 4-semialdehyde + NADP+ + phosphate (EC 1.2.1.11)
AO353_17160 +0.0
AO353_20640 N.D.
AO353_20645 -0.5
Homoserine dehydrogenase (in reverse):
L-aspartate 4-semialdehyde + NAD(P)H + H+→L-homoserine + NAD(P)+ (EC 1.1.1.3)
AO353_04355 -1.6
Sulfite reductase (NADPH) (in reverse):
sulfite + 3 NADPH + 5 H+→hydrogen sulfide + 3 NADP+ + 3 H2O (EC 1.8.1.2)
AO353_05465 +0.0
AO353_20080 -0.2
AO353_22800 -3.7
AO353_22865 +0.2
Homoserine O-acetyltransferase:
L-homoserine + acetyl-CoA→O-acetyl-L-homoserine + coenzyme A (EC 2.3.1.31)
AO353_08180 -2.9
AO353_08185 -3.2
O-acetylhomoserine aminocarboxypropyltransferase:
O-acetyl-L-homoserine + hydrogen sulfide→L-homocysteine + acetate + H+ (EC 2.5.1.49)
AO353_20695 -4.0
5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase:
L-homocysteine + 5-methyltetrahydropteroyl tri-L-glutamate→L-methionine + tetrahydropteroyl tri-L-glutamate (EC 2.1.1.14)
AO353_16215 +0.0
Methionine synthase:
L-homocysteine + a 5-methyltetrahydrofolate→L-methionine + a tetrahydrofolate (EC 2.1.1.13)
AO353_21615 -0.8

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information