MetaCyc Pathway: polyhydroxybutanoate biosynthesis in Pseudomonas fluorescens FW300-N2E3

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Reactions and Genes L-Isoleucine (C)
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Acetyl-CoA C-acyltransferase:
2 acetyl-CoA→acetoacetyl-CoA + coenzyme A (EC 2.3.1.16; 2.3.1.9)
AO353_00505 -0.3
AO353_01065 +0.2
AO353_01160 -0.3
AO353_05785 +0.1
AO353_13880 +0.0
AO353_17205 -0.3
AO353_25685 -3.7
AO353_27530 -0.1
AO353_27945 -0.4
Acetoacetyl-CoA reductase (in reverse):
acetoacetyl-CoA + NADPH + H+→(3R)-3-hydroxybutanoyl-CoA + NADP+ (EC 1.1.1.36)
AO353_01060 -0.3
(3R)-3-hydroxybutanoyl-CoA + poly-[(R)-3-hydroxybutanoate]→poly-[(R)-3-hydroxybutanoate] + coenzyme A (EC 2.3.1.304)
AO353_04270 -0.0

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information