MetaCyc Pathway: oleate β-oxidation in Pseudomonas stutzeri RCH2

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Reactions and Genes Furfuryl Alcohol 0.05 vol%
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Long-chain-fatty-acid--CoA ligase:
oleate + ATP + coenzyme A→oleoyl-CoA + AMP + diphosphate
(EC 6.2.1.3)
Psest_1086 -0.7
Psest_1334 +0.7
Psest_1335 +1.7
Psest_2477 -0.3
oleoyl-CoA + an oxidized electron-transfer flavoprotein + H+→(2E,9Z)-octadec-2,9-enoyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.8)
No genes
Enoyl-CoA hydratase (in reverse):
(2E,9Z)-octadec-2,9-enoyl-CoA + H2O→(S)-3-hydroxy-(9Z)-octadecenoyl-CoA
(EC 4.2.1.17)
Psest_2076 -0.1
Psest_2437 -0.5
Psest_2439 +0.6
Psest_2654 N.D.
Psest_2720 -0.1
Psest_3109 -0.2
Psest_3110 -0.6
Psest_3800 -0.4
Psest_3969 +1.1
Psest_4235 -0.1
3-hydroxyacyl-CoA dehydrogenase:
(S)-3-hydroxy-(9Z)-octadecenoyl-CoA + NAD+→3-oxo-(9Z)-octadecenoyl-CoA + NADH + H+
(EC 1.1.1.35)
Psest_2654 N.D.
Psest_3800 -0.4
Psest_3969 +1.1
Psest_4235 -0.1
Acetyl-CoA C-acyltransferase (in reverse):
3-oxo-(9Z)-octadecenoyl-CoA + coenzyme A→acetyl-CoA + (7Z)-hexadecenoyl-CoA
(EC 2.3.1.16)
Psest_1076 -0.8
Psest_1829 -0.2
Psest_2446 -0.0
Psest_2476 -0.2
Psest_2653 N.D.
Psest_3070 -0.6
(7Z)-hexadecenoyl-CoA + an oxidized electron-transfer flavoprotein + H+→(2E,7Z)-hexadec-2,7-dienoyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.8)
No genes
Enoyl-CoA hydratase (in reverse):
(2E,7Z)-hexadec-2,7-dienoyl-CoA + H2O→(S)-3-hydroxy-(7Z)-hexadecenoyl-CoA
(EC 4.2.1.17)
Psest_2076 -0.1
Psest_2437 -0.5
Psest_2439 +0.6
Psest_2654 N.D.
Psest_2720 -0.1
Psest_3109 -0.2
Psest_3110 -0.6
Psest_3800 -0.4
Psest_3969 +1.1
Psest_4235 -0.1
3-hydroxyacyl-CoA dehydrogenase:
(S)-3-hydroxy-(7Z)-hexadecenoyl-CoA + NAD+→3-oxo-(7Z)-hexadecenoyl-CoA + NADH + H+
(EC 1.1.1.35)
Psest_2654 N.D.
Psest_3800 -0.4
Psest_3969 +1.1
Psest_4235 -0.1
Acetyl-CoA C-acyltransferase (in reverse):
3-oxo-(7Z)-hexadecenoyl-CoA + coenzyme A→acetyl-CoA + (5Z)-tetradecenoyl-CoA
(EC 2.3.1.16)
Psest_1076 -0.8
Psest_1829 -0.2
Psest_2446 -0.0
Psest_2476 -0.2
Psest_2653 N.D.
Psest_3070 -0.6
(5Z)-tetradecenoyl-CoA + an oxidized electron-transfer flavoprotein + H+→(2E,5Z)-tetradec-2,5-enoyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.8)
No genes
Dodecenoyl-CoA isomerase:
(2E,5Z)-tetradec-2,5-enoyl-CoA→(3E,5Z)-tetradeca-3,5-dienoyl-CoA
(EC 5.3.3.8)
Psest_2654 N.D.
Psest_3969 +1.1
Psest_4235 -0.1
Enoyl-CoA hydratase (in reverse):
(2E,5Z)-tetradec-2,5-enoyl-CoA + H2O→(S)-3-hydroxy-(5Z)-tetradecenoyl-CoA
(EC 4.2.1.17)
Psest_2076 -0.1
Psest_2437 -0.5
Psest_2439 +0.6
Psest_2654 N.D.
Psest_2720 -0.1
Psest_3109 -0.2
Psest_3110 -0.6
Psest_3800 -0.4
Psest_3969 +1.1
Psest_4235 -0.1
Long-chain-3-hydroxyacyl-CoA dehydrogenase:
(S)-3-hydroxy-(5Z)-tetradecenoyl-CoA + NAD+→3-oxo-(5Z)-tetradecenoyl-CoA + NADH + H+
(EC 1.1.1.211; 1.1.1.35)
Psest_2654 N.D.
Psest_3800 -0.4
Psest_3969 +1.1
Psest_4235 -0.1
(3E,5Z)-tetradeca-3,5-dienoyl-CoA + H2O→(3E,5Z)-tetradeca-3,5-dienoate + coenzyme A + H+
Psest_3499 +0.2
Acetyl-CoA C-acyltransferase (in reverse):
3-oxo-(5Z)-tetradecenoyl-CoA + coenzyme A→acetyl-CoA + (3Z)-dodec-3-enoyl-CoA
(EC 2.3.1.16)
Psest_1076 -0.8
Psest_1829 -0.2
Psest_2446 -0.0
Psest_2476 -0.2
Psest_2653 N.D.
Psest_3070 -0.6
Dodecenoyl-CoA isomerase:
(3Z)-dodec-3-enoyl-CoA→(2E)-dodec-2-enoyl-CoA
(EC 5.3.3.8)
Psest_2654 N.D.
Psest_3969 +1.1
Psest_4235 -0.1
Long-chain-enoyl-CoA hydratase (in reverse):
(2E)-dodec-2-enoyl-CoA + H2O→(S)-3-hydroxydodecanoyl-CoA
(EC 4.2.1.74)
No genes
Long-chain-3-hydroxyacyl-CoA dehydrogenase:
(S)-3-hydroxydodecanoyl-CoA + NAD+→3-oxododecanoyl-CoA + NADH + H+
(EC 1.1.1.211; 1.1.1.35)
Psest_2654 N.D.
Psest_3800 -0.4
Psest_3969 +1.1
Psest_4235 -0.1
Acetyl-CoA C-acyltransferase (in reverse):
3-oxododecanoyl-CoA + coenzyme A→acetyl-CoA + decanoyl-CoA
(EC 2.3.1.16)
Psest_1076 -0.8
Psest_1829 -0.2
Psest_2446 -0.0
Psest_2476 -0.2
Psest_2653 N.D.
Psest_3070 -0.6
decanoyl-CoA + an oxidized electron-transfer flavoprotein + H+→(2E)-dec-2-enoyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.7)
Psest_2728 -0.1
Psest_3968 +0.1
Psest_0417 +4.0
Psest_0419 -0.5
Psest_0539 -0.6
Long-chain-enoyl-CoA hydratase (in reverse):
(2E)-dec-2-enoyl-CoA + H2O→(S)-3-hydroxydecanoyl-CoA
(EC 4.2.1.74)
Psest_2654 N.D.
Psest_3969 +1.1
Psest_4235 -0.1
3-hydroxyacyl-CoA dehydrogenase:
(S)-3-hydroxydecanoyl-CoA + NAD+→3-oxodecanoyl-CoA + NADH + H+
(EC 1.1.1.35)
Psest_2654 N.D.
Psest_3109 -0.2
Psest_3800 -0.4
Psest_3969 +1.1
Psest_4235 -0.1
Acetyl-CoA C-acyltransferase (in reverse):
3-oxodecanoyl-CoA + coenzyme A→acetyl-CoA + octanoyl-CoA
(EC 2.3.1.16)
Psest_1076 -0.8
Psest_1829 -0.2
Psest_2446 -0.0
Psest_2476 -0.2
Psest_2653 N.D.
Psest_3070 -0.6
octanoyl-CoA + an oxidized electron-transfer flavoprotein + H+→(2E)-oct-2-enoyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.7)
Psest_3968 +0.1
Psest_0417 +4.0
Psest_0419 -0.5
Psest_0539 -0.6
Long-chain-enoyl-CoA hydratase (in reverse):
(2E)-oct-2-enoyl-CoA + H2O→(3S)-3-hydroxyoctanoyl-CoA
(EC 4.2.1.74)
No genes
3-hydroxyacyl-CoA dehydrogenase:
(3S)-3-hydroxyoctanoyl-CoA + NAD+→3-oxooctanoyl-CoA + NADH + H+
(EC 1.1.1.35)
Psest_2654 N.D.
Psest_3800 -0.4
Psest_3969 +1.1
Psest_4235 -0.1
Acetyl-CoA C-acyltransferase (in reverse):
3-oxooctanoyl-CoA + coenzyme A→acetyl-CoA + hexanoyl-CoA
(EC 2.3.1.16)
Psest_1076 -0.8
Psest_1829 -0.2
Psest_2446 -0.0
Psest_2476 -0.2
Psest_2653 N.D.
Psest_3070 -0.6
hexanoyl-CoA + an oxidized electron-transfer flavoprotein + H+→(2E)-hex-2-enoyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.7)
Psest_3968 +0.1
Psest_0417 +4.0
Psest_0419 -0.5
Psest_0539 -0.6
Long-chain-enoyl-CoA hydratase (in reverse):
(2E)-hex-2-enoyl-CoA + H2O→(S)-3-hydroxyhexanoyl-CoA
(EC 4.2.1.74)
Psest_2654 N.D.
Psest_3969 +1.1
Psest_4235 -0.1
3-hydroxyacyl-CoA dehydrogenase:
(S)-3-hydroxyhexanoyl-CoA + NAD+→3-oxohexanoyl-CoA + NADH + H+
(EC 1.1.1.35)
Psest_2654 N.D.
Psest_3109 -0.2
Psest_3800 -0.4
Psest_3969 +1.1
Psest_4235 -0.1
Acetyl-CoA C-acyltransferase (in reverse):
3-oxohexanoyl-CoA + coenzyme A→acetyl-CoA + butanoyl-CoA
(EC 2.3.1.16; 2.3.1.9)
Psest_1076 -0.8
Psest_1829 -0.2
Psest_2446 -0.0
Psest_2476 -0.2
Psest_2653 N.D.
Psest_3070 -0.6
butanoyl-CoA + an oxidized electron-transfer flavoprotein + H+→crotonyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.1)
Psest_0399 -0.4
crotonyl-CoA + H2O→(S)-3-hydroxybutanoyl-CoA (EC 4.2.1.150)
Psest_2654 N.D.
Psest_3969 +1.1
Psest_4235 -0.1
3-hydroxyacyl-CoA dehydrogenase:
(S)-3-hydroxybutanoyl-CoA + NAD+→acetoacetyl-CoA + NADH + H+
(EC 1.1.1.35)
Psest_2654 N.D.
Psest_3109 -0.2
Psest_3800 -0.4
Psest_3969 +1.1
Psest_4235 -0.1
Acetyl-CoA C-acyltransferase (in reverse):
acetoacetyl-CoA + coenzyme A→2 acetyl-CoA
(EC 2.3.1.16; 2.3.1.9)
Psest_1076 -0.8
Psest_1829 -0.2
Psest_2446 -0.0
Psest_2476 -0.2
Psest_2653 N.D.
Psest_3070 -0.6

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information