MetaCyc Pathway: TCA cycle I (prokaryotic) in Pseudomonas stutzeri RCH2

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Reactions and Genes Vanillin 2 mM
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2-oxoglutarate + coenzyme A + NAD+→succinyl-CoA + CO2 + NADH (EC 1.2.1.105)
Psest_2218 +0.5
Succinate--CoA ligase (ADP-forming) (in reverse):
succinyl-CoA + ADP + phosphate→succinate + ATP + coenzyme A (EC 6.2.1.5)
Psest_2490 -2.1
Psest_2491 -2.0
Succinate dehydrogenase (ubiquinone):
succinate[in] + an electron-transfer quinone→fumarate[in] + an electron-transfer quinol (EC 1.3.5.1)
Psest_2495 N.D.
Psest_2496 N.D.
Psest_2497 N.D.
Psest_2498 N.D.
Fumarate hydratase (in reverse):
fumarate + H2O→(S)-malate (EC 4.2.1.2)
Psest_2682 -2.3
Psest_3193 N.D.
Malate dehydrogenase:
(S)-malate + NAD+→oxaloacetate + NADH + H+ (EC 1.1.1.37; 1.1.1.38)
No genes
(S)-malate[in] + an electron-transfer quinone→oxaloacetate[in] + an electron-transfer quinol (EC 1.1.5.4)
Psest_3238 N.D.
Citrate (Si)-synthase:
oxaloacetate + acetyl-CoA + H2O→citrate + coenzyme A + H+ (EC 2.3.3.1; 2.3.3.16; 2.3.3.3)
Psest_2499 N.D.
Aconitate hydratase:
citrate→cis-aconitate + H2O (EC 4.2.1.3)
Psest_2309 N.D.
Psest_2319 N.D.
Psest_2521 -0.3
Aconitate hydratase:
cis-aconitate + H2O→D-threo-isocitrate (EC 4.2.1.3)
Psest_2309 N.D.
Psest_2319 N.D.
Psest_2521 -0.3
Isocitrate dehydrogenase (NADP(+)):
D-threo-isocitrate + NADP+→2-oxoglutarate + CO2 + NADPH (EC 1.1.1.42)
Psest_2023 +0.2
Psest_2024 +0.3

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information