MetaCyc Pathway: pyruvate fermentation to isobutanol (engineered) in Klebsiella michiganensis M5al

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Reactions and Genes a-Cyclodextrin (C)
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Acetolactate synthase:
2 pyruvate + H+→(S)-2-acetolactate + CO2 (EC 2.2.1.6)
BWI76_RS00960 -1.1
BWI76_RS00965 -2.7
BWI76_RS04695 +0.1
BWI76_RS04700 -0.1
BWI76_RS12900 -0.1
BWI76_RS16750 -0.3
BWI76_RS27810 -0.2
BWI76_RS27815 -1.0
Ketol-acid reductoisomerase (in reverse):
(S)-2-acetolactate + NADPH + H+→(2R)-2,3-dihydroxy-3-methylbutanoate + NADP+ (EC 1.1.1.86)
BWI76_RS00990 -4.6
Dihydroxy-acid dehydratase:
(2R)-2,3-dihydroxy-3-methylbutanoate→3-methyl-2-oxobutanoate + H2O (EC 4.2.1.9)
BWI76_RS00975 -4.3
Branched-chain-2-oxoacid decarboxylase:
3-methyl-2-oxobutanoate + H+→isobutanal + CO2 (EC 4.1.1.72)
No genes
Alcohol dehydrogenase (in reverse):
isobutanal + NADH + H+→isobutanol + NAD+ (EC 1.1.1.1)
BWI76_RS00645 +0.1
BWI76_RS02835 -0.0
BWI76_RS03140 -0.1
BWI76_RS03320 +0.0
BWI76_RS12395 +1.4
BWI76_RS13800 -0.2
BWI76_RS15240 -0.0
BWI76_RS17250 -0.2
BWI76_RS25510 +0.0
BWI76_RS27960 +0.0

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information