MetaCyc Pathway: ammonia assimilation cycle III in Pseudomonas fluorescens FW300-N1B4

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Reactions and Genes Putrescine (C)
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Glutamate synthase (NADPH):
L-glutamine + H2O→ammonia + L-glutamate + H+ (EC 1.4.1.13; 1.4.1.14; 1.4.7.1; 2.6.1.85; 3.5.1.2; 3.5.1.38; 4.1.3.27; 4.3.2.10; 4.3.3.6; 6.3.4.2; 6.3.5.13; 6.3.5.2; 6.3.5.4; 6.3.5.5; 6.3.5.6; 6.3.5.7)
Pf1N1B4_1100 N.D.
Pf1N1B4_1101 N.D.
Pf1N1B4_1102 N.D.
Pf1N1B4_1172 N.D.
Pf1N1B4_1173 N.D.
Pf1N1B4_1533 -1.7
Pf1N1B4_1534 -1.5
Pf1N1B4_2545 -3.0
Pf1N1B4_2547 -3.0
Pf1N1B4_2646 +0.0
Pf1N1B4_290 +0.7
Pf1N1B4_3102 N.D.
Pf1N1B4_3209 -0.1
Pf1N1B4_3229 +0.3
Pf1N1B4_3412 +0.2
Pf1N1B4_3592 +0.3
Pf1N1B4_3722 +1.0
Pf1N1B4_374 -0.1
Pf1N1B4_3797 +0.2
Pf1N1B4_3814 +0.5
Pf1N1B4_3940 N.D.
Pf1N1B4_4165 -0.1
Pf1N1B4_4436 -0.4
Pf1N1B4_4516 -0.2
Pf1N1B4_6037 -0.5
Pf1N1B4_706 N.D.
Pf1N1B4_839 N.D.
Pf1N1B4_875 -0.9
Glutamate synthase (NADPH) (in reverse):
ammonia + 2-oxoglutarate + NADPH + 2 H+→L-glutamate + NADP+ + H2O (EC 1.4.1.13; 1.4.1.14)
Pf1N1B4_1533 -1.7
Pf1N1B4_1534 -1.5
Pf1N1B4_2646 +0.0
Pf1N1B4_290 +0.7
Pf1N1B4_3209 -0.1
Pf1N1B4_3229 +0.3
Pf1N1B4_3592 +0.3
Pf1N1B4_3722 +1.0
Pf1N1B4_3940 N.D.
Pf1N1B4_4516 -0.2
Pf1N1B4_706 N.D.
Pf1N1B4_875 -0.9
Glutamate--ammonia ligase:
L-glutamate + ammonium + ATP→L-glutamine + ADP + H+ + phosphate (EC 6.3.1.2)
Pf1N1B4_1606 N.D.
Pf1N1B4_2254 -2.4
Pf1N1B4_2256 -0.6
Pf1N1B4_3342 +0.0
Pf1N1B4_3464 +0.2
Pf1N1B4_4245 +0.2
Pf1N1B4_4354 -0.8
Pf1N1B4_4826 +0.7
Pf1N1B4_5853 +0.9

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information