MetaCyc Pathway: (R)- and (S)-3-hydroxybutanoate biosynthesis (engineered) in Acidovorax sp. GW101-3H11

Reactions and Genes	L-Isoleucine (N)
Acetyl-CoA C-acyltransferase: 2 acetyl-CoA→acetoacetyl-CoA + coenzyme A (EC 2.3.1.16; 2.3.1.9)
Ac3H11_178	-0.0
Ac3H11_1916	-0.1
Ac3H11_1932	+0.0
Ac3H11_2303	-0.3
Ac3H11_2522	+0.2
Ac3H11_2740	N.D.
Ac3H11_2769	-0.4
Ac3H11_2994	-3.0
Ac3H11_3407	+0.3
Ac3H11_3408	+0.1
Ac3H11_3920	-0.6
3-hydroxyacyl-CoA dehydrogenase (in reverse): acetoacetyl-CoA + NADH + H+→(S)-3-hydroxybutanoyl-CoA + NAD+ (EC 1.1.1.35)
Ac3H11_1056	N.D.
Ac3H11_1914	-0.2
Ac3H11_1931	-0.1
Ac3H11_2523	-0.2
Ac3H11_2606	-5.2
Ac3H11_2741	-0.3
Ac3H11_4075	-0.1
Ac3H11_4658	-0.2
Acetoacetyl-CoA reductase (in reverse): acetoacetyl-CoA + NADPH + H+→(3R)-3-hydroxybutanoyl-CoA + NADP+ (EC 1.1.1.36)
Ac3H11_2302	+1.0
(S)-3-hydroxybutanoyl-CoA + H2O→(S)-3-hydroxybutanoate + coenzyme A + H+
No genes
(3R)-3-hydroxybutanoyl-CoA + H2O→(R)-3-hydroxybutanoate + coenzyme A + H+
No genes

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information