MetaCyc Pathway: L-valine degradation I in Pseudomonas fluorescens FW300-N1B4

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Reactions and Genes L-Valine (C)
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Branched-chain-amino-acid transaminase:
L-valine + 2-oxoglutarate→3-methyl-2-oxobutanoate + L-glutamate (EC 2.6.1.42)
Pf1N1B4_4476 -2.7
2-oxoisovalerate dehydrogenase (acylating):
3-methyl-2-oxobutanoate + coenzyme A + NAD+→isobutanoyl-CoA + CO2 + NADH (EC 1.2.1.25)
No genes
isobutanoyl-CoA + an oxidized electron-transfer flavoprotein + H+→methylacrylyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.5)
Pf1N1B4_4787 -0.4
methylacrylyl-CoA + H2O→(S)-3-hydroxy-isobutanoyl-CoA (EC 4.2.1.150)
Pf1N1B4_1787 +0.0
Pf1N1B4_267 -0.2
Pf1N1B4_3308 +0.0
Pf1N1B4_3903 N.D.
Pf1N1B4_4777 -0.1
Pf1N1B4_4788 N.D.
Pf1N1B4_679 +0.3
3-hydroxyisobutyryl-CoA hydrolase:
(S)-3-hydroxy-isobutanoyl-CoA + H2O→(S)-3-hydroxy-isobutanoate + coenzyme A + H+ (EC 3.1.2.4)
Pf1N1B4_4790 -3.4
3-hydroxyisobutyrate dehydrogenase:
(S)-3-hydroxy-isobutanoate + NAD+→(S)-methylmalonate-semialdehyde + NADH + H+ (EC 1.1.1.31)
Pf1N1B4_1228 -2.5
Pf1N1B4_1787 +0.0
Pf1N1B4_3796 +0.4
Pf1N1B4_3903 N.D.
Pf1N1B4_5077 -0.5
(S)-3-amino-2-methylpropionate transaminase (in reverse):
(S)-methylmalonate-semialdehyde + L-glutamate→(S)-3-amino-2-methylpropanoate + 2-oxoglutarate (EC 2.6.1.22)
No genes
Methylmalonate-semialdehyde dehydrogenase (acylating):
(S)-methylmalonate-semialdehyde + coenzyme A + NAD+ + H2O→propanoyl-CoA + hydrogen carbonate + NADH + H+ (EC 1.2.1.27)
Pf1N1B4_1229 -2.7
Pf1N1B4_1238 -0.4
Pf1N1B4_4277 +0.1

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information