MetaCyc Pathway: mixed acid fermentation in Pseudomonas fluorescens FW300-N1B4

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Reactions and Genes L-Valine (C)
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Phosphoenolpyruvate carboxylase (in reverse):
phosphoenolpyruvate + hydrogen carbonate→oxaloacetate + phosphate (EC 4.1.1.31)
Pf1N1B4_3051 -0.2
Pyruvate kinase (in reverse):
phosphoenolpyruvate + ADP + H+→pyruvate + ATP (EC 2.7.1.40)
Pf1N1B4_3657 -0.6
Pf1N1B4_678 +0.4
D-lactate dehydrogenase (in reverse):
pyruvate + NADH + H+→(R)-lactate + NAD+ (EC 1.1.1.28)
Pf1N1B4_3500 -0.2
Malate dehydrogenase (in reverse):
oxaloacetate + NADH + H+→(S)-malate + NAD+ (EC 1.1.1.37; 1.1.1.38)
No genes
Formate C-acetyltransferase (in reverse):
pyruvate + coenzyme A→acetyl-CoA + formate (EC 2.3.1.54)
No genes
Acetaldehyde dehydrogenase (acetylating) (in reverse):
acetyl-CoA + NADH + H+→acetaldehyde + coenzyme A + NAD+ (EC 1.2.1.10)
Pf1N1B4_4506 -0.9
Citrate (Si)-synthase:
acetyl-CoA + oxaloacetate + H2O→citrate + coenzyme A + H+ (EC 2.3.3.1; 2.3.3.16; 2.3.3.3)
Pf1N1B4_3676 N.D.
formate + H+→CO2 + H2
No genes
Fumarate hydratase:
(S)-malate→fumarate + H2O (EC 4.2.1.2)
Pf1N1B4_1088 N.D.
Pf1N1B4_367 +0.2
Pf1N1B4_683 -0.6
Phosphate acetyltransferase:
acetyl-CoA + phosphate→acetyl phosphate + coenzyme A (EC 2.3.1.8)
Pf1N1B4_1054 +0.3
Pf1N1B4_1055 +0.2
Acetate kinase (in reverse):
acetyl phosphate + ADP→acetate + ATP (EC 2.7.2.1; 2.7.2.15)
Pf1N1B4_879 +0.5
Aconitate hydratase:
citrate→cis-aconitate + H2O (EC 4.2.1.3)
Pf1N1B4_3821 -2.6
Pf1N1B4_3888 -1.7
Pf1N1B4_4564 N.D.
Alcohol dehydrogenase (in reverse):
acetaldehyde + NADH + H+→ethanol + NAD+ (EC 1.1.1.1)
Pf1N1B4_2542 +0.0
Pf1N1B4_3108 -0.2
Pf1N1B4_3270 -0.4
Pf1N1B4_395 -0.1
Pf1N1B4_4176 -0.4
Pf1N1B4_4243 +0.2
Pf1N1B4_4506 -0.9
Pf1N1B4_4898 N.D.
Pf1N1B4_5026 N.D.
Succinate dehydrogenase (ubiquinone):
fumarate[in] + a menaquinol→succinate[in] + a menaquinone (EC 1.3.5.1)
No genes
Aconitate hydratase:
cis-aconitate + H2O→D-threo-isocitrate (EC 4.2.1.3)
Pf1N1B4_3821 -2.6
Pf1N1B4_3888 -1.7
Pf1N1B4_4564 N.D.
Isocitrate dehydrogenase (NADP(+)):
D-threo-isocitrate + NADP+→CO2 + 2-oxoglutarate + NADPH (EC 1.1.1.42)
Pf1N1B4_4050 N.D.
Pf1N1B4_4051 +0.1
Pf1N1B4_4052 +0.3

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information