MetaCyc Pathway: L-glutamate and L-glutamine biosynthesis in Pseudomonas fluorescens FW300-N2E3

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Reactions and Genes acetate 17 mM
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Glutamate dehydrogenase (NAD(P)(+)) (in reverse):
2-oxoglutarate + ammonium + NAD(P)H + H+→L-glutamate + NAD(P)+ + H2O (EC 1.4.1.3)
No genes
Glutamate synthase (ferredoxin) (in reverse):
2-oxoglutarate + L-glutamine + 2 H+ + 2 a reduced ferredoxin [iron-sulfur] cluster→2 L-glutamate + 2 an oxidized ferredoxin [iron-sulfur] cluster (EC 1.4.7.1)
AO353_16160 -0.0
Glutamate synthase (NADH) (in reverse):
2-oxoglutarate + L-glutamine + NADH + H+→2 L-glutamate + NAD+ (EC 1.4.1.14)
AO353_12520 -0.1
AO353_26575 -0.1
Glutamate synthase (NADPH):
L-glutamine + H2O→L-glutamate + ammonia + H+ (EC 1.4.1.13; 1.4.1.14; 1.4.7.1; 2.6.1.85; 3.5.1.2; 3.5.1.38; 4.1.3.27; 4.3.2.10; 4.3.3.6; 6.3.4.2; 6.3.5.13; 6.3.5.2; 6.3.5.4; 6.3.5.5; 6.3.5.6; 6.3.5.7)
AO353_00925 -0.2
AO353_01150 -0.1
AO353_02570 -0.4
AO353_03130 -0.4
AO353_03905 +0.1
AO353_04395 N.D.
AO353_05255 N.D.
AO353_05260 N.D.
AO353_05265 N.D.
AO353_05630 N.D.
AO353_05635 N.D.
AO353_06730 +0.0
AO353_07220 -0.0
AO353_07230 +0.0
AO353_10550 +1.7
AO353_12515 +0.0
AO353_12520 -0.1
AO353_15955 N.D.
AO353_16160 -0.0
AO353_16550 N.D.
AO353_17090 +0.3
AO353_17175 -0.1
AO353_19025 +0.5
AO353_20130 -0.2
AO353_20805 -0.0
AO353_26575 -0.1
AO353_26790 +0.1
Glutamate--ammonia ligase:
L-glutamate + ammonium + ATP→L-glutamine + ADP + H+ + phosphate (EC 6.3.1.2)
AO353_02900 +0.1
AO353_08590 -0.4
AO353_08595 +0.1
AO353_12170 N.D.
AO353_24780 +0.1
AO353_24910 -0.1
AO353_27475 +0.1
AO353_27900 +0.2
AO353_29300 -0.1
Glutamate dehydrogenase (NADP(+)) (in reverse):
2-oxoglutarate + ammonium + NADPH + H+→L-glutamate + NADP+ + H2O (EC 1.4.1.4)
AO353_12520 -0.1
Glutamate synthase (NADPH) (in reverse):
2-oxoglutarate + ammonia + NADPH + 2 H+→L-glutamate + NADP+ + H2O (EC 1.4.1.13; 1.4.1.14)
AO353_02570 -0.4
AO353_03905 +0.1
AO353_04395 N.D.
AO353_06730 +0.0
AO353_12515 +0.0
AO353_12520 -0.1
AO353_17090 +0.3
AO353_17175 -0.1
AO353_19025 +0.5
AO353_20130 -0.2
AO353_26575 -0.1

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information