MetaCyc Pathway: pyruvate fermentation to isobutanol (engineered) in Pseudomonas fluorescens FW300-N2E3

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Reactions and Genes Furfuryl Alcohol 0.25 vol%
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Acetolactate synthase:
2 pyruvate + H+→(S)-2-acetolactate + CO2 (EC 2.2.1.6)
AO353_03865 -0.2
AO353_14495 -0.2
AO353_14500 -0.0
AO353_23145 +0.1
AO353_24845 -0.0
Ketol-acid reductoisomerase (in reverse):
(S)-2-acetolactate + NADPH + H+→(2R)-2,3-dihydroxy-3-methylbutanoate + NADP+ (EC 1.1.1.86)
AO353_14505 +0.1
Dihydroxy-acid dehydratase:
(2R)-2,3-dihydroxy-3-methylbutanoate→3-methyl-2-oxobutanoate + H2O (EC 4.2.1.9)
AO353_08345 +0.1
Branched-chain-2-oxoacid decarboxylase:
3-methyl-2-oxobutanoate + H+→isobutanal + CO2 (EC 4.1.1.72)
No genes
Alcohol dehydrogenase (in reverse):
isobutanal + NADH + H+→isobutanol + NAD+ (EC 1.1.1.1)
AO353_01250 -0.1
AO353_07245 -0.0
AO353_16580 -1.2
AO353_17425 -0.1
AO353_19865 +0.0
AO353_22415 -0.2
AO353_24900 +0.3
AO353_28025 +0.0

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information