MetaCyc Pathway: formaldehyde oxidation II (glutathione-dependent) in Pseudomonas fluorescens FW300-N2E3

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Reactions and Genes methylglyoxal 0.009 vol%
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S-(hydroxymethyl)glutathione synthase (in reverse):
formaldehyde + glutathione→S-(hydroxymethyl)glutathione (spontaneous) (EC 4.4.1.22)
AO353_25200 -0.2
S-(hydroxymethyl)glutathione dehydrogenase:
S-(hydroxymethyl)glutathione + NAD(P)+→S-formylglutathione + NAD(P)H + H+ (EC 1.1.1.284)
AO353_16580 -1.6
S-formylglutathione hydrolase:
S-formylglutathione + H2O→formate + glutathione + H+ (EC 3.1.2.12)
AO353_16585 -2.7

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information