MetaCyc Pathway: glycerol degradation to butanol in Sphingomonas koreensis DSMZ 15582

Add experiment(s):


Reactions and Genes Vanillin 0.25 mM
remove
Glycerol dehydrogenase:
glycerol + NAD+→dihydroxyacetone + NADH + H+ (EC 1.1.1.6)
Ga0059261_2879 -0.0
Glycerone kinase:
dihydroxyacetone + ATP→glycerone phosphate + ADP + H+ (EC 2.7.1.29)
No genes
Triose-phosphate isomerase (in reverse):
glycerone phosphate→D-glyceraldehyde 3-phosphate (EC 5.3.1.1)
Ga0059261_0254 N.D.
Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating):
D-glyceraldehyde 3-phosphate + NAD+ + phosphate→3-phospho-D-glyceroyl phosphate + NADH + H+ (EC 1.2.1.12)
Ga0059261_0730 N.D.
Phosphoglycerate kinase (in reverse):
3-phospho-D-glyceroyl phosphate + ADP→3-phospho-D-glycerate + ATP (EC 2.7.2.3)
Ga0059261_0728 N.D.
3-phospho-D-glycerate→2-phospho-D-glycerate (EC 5.4.2.12)
Ga0059261_3169 +0.0
Phosphopyruvate hydratase:
2-phospho-D-glycerate→phosphoenolpyruvate + H2O (EC 4.2.1.11)
Ga0059261_0014 +0.2
Pyruvate kinase (in reverse):
phosphoenolpyruvate + ADP + H+→pyruvate + ATP (EC 2.7.1.40)
Ga0059261_0685 +0.1
Pyruvate synthase:
pyruvate + coenzyme A + 2 an oxidized ferredoxin [iron-sulfur] cluster→acetyl-CoA + CO2 + H+ + 2 a reduced ferredoxin [iron-sulfur] cluster (EC 1.2.7.1)
No genes
Acetyl-CoA C-acyltransferase:
2 acetyl-CoA→acetoacetyl-CoA + coenzyme A (EC 2.3.1.16; 2.3.1.9)
Ga0059261_0982 -0.3
Ga0059261_1483 -0.4
Ga0059261_2108 -0.0
Ga0059261_2160 +0.0
Ga0059261_2808 -0.1
Ga0059261_2835 -0.0
Ga0059261_2867 +0.0
Ga0059261_2877 +0.1
Ga0059261_2888 +0.1
Ga0059261_3284 -0.4
3-hydroxyacyl-CoA dehydrogenase (in reverse):
acetoacetyl-CoA + NADH + H+→(S)-3-hydroxybutanoyl-CoA + NAD+ (EC 1.1.1.35)
Ga0059261_0837 -0.1
Ga0059261_2534 -0.7
Ga0059261_2816 +0.1
Ga0059261_2827 -0.1
(S)-3-hydroxybutanoyl-CoA→crotonyl-CoA + H2O (EC 4.2.1.150)
Ga0059261_0515 +0.2
Ga0059261_0837 -0.1
Ga0059261_2159 -0.4
Ga0059261_2668 -0.0
Ga0059261_2803 +0.0
Ga0059261_2815 +0.0
Ga0059261_2818 +0.1
Ga0059261_2827 -0.1
Ga0059261_2840 +0.1
Ga0059261_2847 -0.2
Ga0059261_2858 -0.0
Ga0059261_2861 +0.0
Ga0059261_2887 +0.0
Ga0059261_2902 -0.1
Ga0059261_2908 +0.0
Ga0059261_3685 -2.4
Ga0059261_3986 +0.1
Ga0059261_4214 -0.8
Ga0059261_4222 -0.1
crotonyl-CoA[cytosol] + 2 NADH[cytosol] + 2 an oxidized ferredoxin [iron-sulfur] cluster[cytosol]→butanoyl-CoA[cytosol] + 2 NAD+[cytosol] + 2 a reduced ferredoxin [iron-sulfur] cluster[cytosol] (EC 1.3.1.109)
No genes
Butanal dehydrogenase (in reverse):
butanoyl-CoA + NAD(P)H + H+→1-butanal + coenzyme A + NAD(P)+ (EC 1.2.1.57)
No genes
Alcohol dehydrogenase (NADP(+)) (in reverse):
1-butanal + NADPH + H+→butan-1-ol + NADP+ (EC 1.1.1.2)
No genes
1-butanal + NADH + H+→butan-1-ol + NAD+
No genes

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information