MetaCyc Pathway: L-isoleucine degradation I in Sphingomonas koreensis DSMZ 15582

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Reactions and Genes Vanillin 0.25 mM
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Branched-chain-amino-acid transaminase:
L-isoleucine + 2-oxoglutarate→(3S)-3-methyl-2-oxopentanoate + L-glutamate (EC 2.6.1.42)
Ga0059261_3811 -0.7
2-oxoisovalerate dehydrogenase (acylating):
(3S)-3-methyl-2-oxopentanoate + coenzyme A + NAD+→(2S)-2-methylbutanoyl-CoA + CO2 + NADH (EC 1.2.1.25)
No genes
(2S)-2-methylbutanoyl-CoA + an oxidized electron-transfer flavoprotein + H+→(E)-2-methylcrotonoyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.5)
No genes
(E)-2-methylcrotonoyl-CoA + H2O→(2S,3S)-3-hydroxy-2-methylbutanoyl-CoA (EC 4.2.1.150)
Ga0059261_0515 +0.2
Ga0059261_0837 -0.1
Ga0059261_2159 -0.4
Ga0059261_2668 -0.0
Ga0059261_2803 +0.0
Ga0059261_2815 +0.0
Ga0059261_2818 +0.1
Ga0059261_2827 -0.1
Ga0059261_2840 +0.1
Ga0059261_2847 -0.2
Ga0059261_2858 -0.0
Ga0059261_2861 +0.0
Ga0059261_2887 +0.0
Ga0059261_2902 -0.1
Ga0059261_2908 +0.0
Ga0059261_3685 -2.4
Ga0059261_3986 +0.1
Ga0059261_4214 -0.8
Ga0059261_4222 -0.1
3-hydroxy-2-methylbutyryl-CoA dehydrogenase:
(2S,3S)-3-hydroxy-2-methylbutanoyl-CoA + NAD+→2-methylacetoacetyl-CoA + NADH + H+ (EC 1.1.1.178)
Ga0059261_0837 -0.1
Ga0059261_2827 -0.1
Acetyl-CoA C-acyltransferase (in reverse):
2-methylacetoacetyl-CoA + coenzyme A→acetyl-CoA + propanoyl-CoA (EC 2.3.1.16)
Ga0059261_0982 -0.3
Ga0059261_1483 -0.4
Ga0059261_2108 -0.0
Ga0059261_2160 +0.0
Ga0059261_2808 -0.1
Ga0059261_2835 -0.0
Ga0059261_2867 +0.0
Ga0059261_2877 +0.1
Ga0059261_2888 +0.1
Ga0059261_3284 -0.4

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information