MetaCyc Pathway: docosahexaenoate biosynthesis III (6-desaturase, mammals) in Sphingomonas koreensis DSMZ 15582

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Reactions and Genes 5-Hydroxymethylfurfural 2.5 mM
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(5Z,8Z,11Z,14Z,17Z)-icosapentaenoyl-CoA + malonyl-CoA + H+→(5Z,8Z,11Z,14Z,17Z)-3-oxodocosapentaenoyl-CoA + CO2 + coenzyme A
No genes
(5Z,8Z,11Z,14Z,17Z)-3-oxodocosapentaenoyl-CoA + NAD(P)H + H+→(3R,5Z,8Z,11Z,14Z,17Z)-3-hydroxydocosapentaenoyl-CoA + NAD(P)+ (EC 1.1.1.330)
No genes
(3R,5Z,8Z,11Z,14Z,17Z)-3-hydroxydocosapentaenoyl-CoA→(2E,7Z,10Z,13Z,16Z,19Z)-docosahexaenoyl-CoA + H2O (EC 4.2.1.134)
No genes
(2E,7Z,10Z,13Z,16Z,19Z)-docosahexaenoyl-CoA + a reduced two electron carrier→(7Z,10Z,13Z,16Z,19Z)-docosapentaenoyl-CoA + an oxidized electron carrier (EC 1.3.1.93)
No genes
(7Z,10Z,13Z,16Z,19Z)-docosapentaenoyl-CoA + malonyl-CoA + H+→(9Z,12Z,15Z,18Z,21Z)-3-oxotetracosapentaenoyl-CoA + CO2 + coenzyme A
No genes
(9Z,12Z,15Z,18Z,21Z)-3-oxotetracosapentaenoyl-CoA + NADPH + H+→(3R,9Z,12Z,15Z,18Z,21Z)-3-hydroxytetracosapentaenoyl-CoA + NADP+ (EC 1.1.1.330)
No genes
(3R,9Z,12Z,15Z,18Z,21Z)-3-hydroxytetracosapentaenoyl-CoA→(2E,9Z,12Z,15Z,18Z,21Z)-tetracosahexaenoyl-CoA + H2O (EC 4.2.1.134)
No genes
(2E,9Z,12Z,15Z,18Z,21Z)-tetracosahexaenoyl-CoA + NADPH + H+→(9Z,12Z,15Z,18Z,21Z)-tetracosapentaenoyl-CoA + NADP+ (EC 1.3.1.93)
No genes
Linoleoyl-CoA desaturase:
(9Z,12Z,15Z,18Z,21Z)-tetracosapentaenoyl-CoA + 2 a ferrocytochrome b5 + dioxygen + 2 H+→(6Z,9Z,12Z,15Z,18Z,21Z)-tetracosahexaenoyl-CoA + 2 a ferricytochrome b5 + 2 H2O
(EC 1.14.19.3)
No genes
Acyl-CoA oxidase:
(6Z,9Z,12Z,15Z,18Z,21Z)-tetracosahexaenoyl-CoA + dioxygen→(2E,6Z,9Z,12Z,15Z,18Z,21Z)-tetracosaheptaenoyl-CoA + hydrogen peroxide
(EC 1.3.3.6)
No genes
Enoyl-CoA hydratase (in reverse):
(2E,6Z,9Z,12Z,15Z,18Z,21Z)-tetracosaheptaenoyl-CoA + H2O→(3S,6Z,9Z,12Z,15Z,18Z,21Z)-3-hydroxytetracosahexaenoyl-CoA
(EC 4.2.1.17)
Ga0059261_0515 +0.1
Ga0059261_0837 -0.2
Ga0059261_2159 -0.1
Ga0059261_2668 +0.0
Ga0059261_2803 -0.1
Ga0059261_2815 -0.1
Ga0059261_2818 -0.0
Ga0059261_2827 -0.0
Ga0059261_2840 -0.4
Ga0059261_2847 +0.0
Ga0059261_2858 +0.1
Ga0059261_2861 -0.1
Ga0059261_2887 -0.2
Ga0059261_2902 +0.0
Ga0059261_2908 -0.0
Ga0059261_3683 -1.0
Ga0059261_3685 -2.3
Ga0059261_3986 +0.0
Ga0059261_4214 -0.6
Ga0059261_4222 +0.0
(3S,6Z,9Z,12Z,15Z,18Z,21Z)-3-hydroxytetracosahexaenoyl-CoA + NAD+→(6Z,9Z,12Z,15Z,18Z,21Z)-3-oxotetracosahexaenoyl-CoA + NADH + H+
No genes
Acetyl-CoA C-acyltransferase (in reverse):
(6Z,9Z,12Z,15Z,18Z,21Z)-3-oxotetracosahexaenoyl-CoA + coenzyme A→(4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoyl-CoA + acetyl-CoA
(EC 2.3.1.16)
Ga0059261_0982 -0.2
Ga0059261_1483 -0.2
Ga0059261_2108 -0.1
Ga0059261_2160 +0.0
Ga0059261_2808 -0.2
Ga0059261_2835 +0.0
Ga0059261_2867 -0.1
Ga0059261_2877 -0.0
Ga0059261_2888 +0.1
Ga0059261_3284 -0.6
(4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoyl-CoA + a glycerolipid→a [glycerolipid]-(4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoate + coenzyme A
No genes

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information