MetaCyc Pathway: methyl ketone biosynthesis (engineered) in Sphingomonas koreensis DSMZ 15582

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Reactions and Genes 5-Hydroxymethylfurfural 2.5 mM
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a fatty acid + ATP + coenzyme A→an acyl-CoA + AMP + diphosphate
No genes
Acyl-CoA oxidase:
a 2,3,4-saturated fatty acyl CoA + dioxygen→a (2E)-2-enoyl-CoA + hydrogen peroxide
(EC 1.3.3.6)
No genes
Enoyl-CoA hydratase (in reverse):
a (2E)-2-enoyl-CoA + H2O→a 2,3,4-saturated (3S)-3-hydroxyacyl-CoA
(EC 4.2.1.17)
Ga0059261_0515 +0.1
Ga0059261_0837 -0.2
Ga0059261_2159 -0.1
Ga0059261_2668 +0.0
Ga0059261_2803 -0.1
Ga0059261_2815 -0.1
Ga0059261_2818 -0.0
Ga0059261_2827 -0.0
Ga0059261_2840 -0.4
Ga0059261_2847 +0.0
Ga0059261_2858 +0.1
Ga0059261_2861 -0.1
Ga0059261_2887 -0.2
Ga0059261_2902 +0.0
Ga0059261_2908 -0.0
Ga0059261_3683 -1.0
Ga0059261_3685 -2.3
Ga0059261_3986 +0.0
Ga0059261_4214 -0.6
Ga0059261_4222 +0.0
3-hydroxyacyl-CoA dehydrogenase:
a 2,3,4-saturated (3S)-3-hydroxyacyl-CoA + NAD+→a 2,3,4-saturated 3-oxoacyl-CoA + NADH + H+
(EC 1.1.1.35)
Ga0059261_0837 -0.2
Ga0059261_2534 -0.6
Ga0059261_2816 -0.1
Ga0059261_2827 -0.0
a 2,3,4-saturated 3-oxoacyl-CoA + H2O→a 3-oxoacid + coenzyme A + H+
No genes
a 3-oxoacid + H+→a methyl ketone + CO2 (spontaneous)
No genes

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information