MetaCyc Pathway: 1-butanol autotrophic biosynthesis (engineered) in Sphingomonas koreensis DSMZ 15582

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Reactions and Genes 5-Hydroxymethylfurfural 2.5 mM
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2 H2O[in] + hν[cytosol] + 2 a plastoquinone + 4 H+[out]→2 a plastoquinol + dioxygen[in] + 4 H+[in] (EC 1.10.3.9)
No genes
a plastoquinol + 2 an oxidized plastocyanin[in] + 2 H+[out]→2 a reduced plastocyanin[in] + a plastoquinone + 4 H+[in] (EC 7.1.1.6)
No genes
a reduced plastocyanin[in] + hν[cytosol] + an oxidized ferredoxin [iron-sulfur] cluster[out]→a reduced ferredoxin [iron-sulfur] cluster[out] + an oxidized plastocyanin[in] (EC 1.97.1.12)
No genes
Ferredoxin--NADP(+) reductase:
2 a reduced ferredoxin [iron-sulfur] cluster[out] + NADP+[out] + H+[out]→NADPH[out] + 2 an oxidized ferredoxin [iron-sulfur] cluster[out] (EC 1.18.1.2)
Ga0059261_3758 N.D.
Ga0059261_3973 -0.0
Glyceraldehyde-3-phosphate dehydrogenase (NADP(+)) (phosphorylating) (in reverse):
3-phospho-D-glyceroyl phosphate + NADPH + H+→D-glyceraldehyde 3-phosphate + NADP+ + phosphate (EC 1.2.1.13)
No genes
Triose-phosphate isomerase:
D-glyceraldehyde 3-phosphate→glycerone phosphate (EC 5.3.1.1)
Ga0059261_0254 N.D.
Fructose-bisphosphate aldolase (in reverse):
glycerone phosphate + D-glyceraldehyde 3-phosphate→β-D-fructofuranose 1,6-bisphosphate (EC 4.1.2.13)
Ga0059261_0725 +0.1
Fructose-bisphosphate aldolase (in reverse):
glycerone phosphate + D-erythrose 4-phosphate→D-sedoheptulose 1,7-bisphosphate (EC 4.1.2.13)
Ga0059261_0725 +0.1
Fructose-bisphosphatase:
β-D-fructofuranose 1,6-bisphosphate + H2O→β-D-fructofuranose 6-phosphate + phosphate (EC 3.1.3.11)
Ga0059261_2707 -2.1
Sedoheptulose-bisphosphatase:
D-sedoheptulose 1,7-bisphosphate + H2O→D-sedoheptulose 7-phosphate + phosphate (EC 3.1.3.37)
No genes
Transketolase:
D-sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate→D-ribose 5-phosphate + D-xylulose 5-phosphate (EC 2.2.1.1)
Ga0059261_0731 N.D.
Ga0059261_2624 +0.2
Ga0059261_2625 -0.0
Transketolase (in reverse):
β-D-fructofuranose 6-phosphate + D-glyceraldehyde 3-phosphate→D-xylulose 5-phosphate + D-erythrose 4-phosphate (EC 2.2.1.1)
Ga0059261_0731 N.D.
Ga0059261_2624 +0.2
Ga0059261_2625 -0.0
Ribose-5-phosphate isomerase:
D-ribose 5-phosphate→D-ribulose 5-phosphate (EC 5.3.1.6)
Ga0059261_1987 N.D.
Ribulose-phosphate 3-epimerase (in reverse):
D-xylulose 5-phosphate→D-ribulose 5-phosphate (EC 5.1.3.1)
Ga0059261_2240 N.D.
Phosphoribulokinase:
D-ribulose 5-phosphate + ATP→D-ribulose-1,5-bisphosphate + ADP + H+ (EC 2.7.1.19)
No genes
Ribulose-bisphosphate carboxylase (in reverse):
D-ribulose-1,5-bisphosphate + CO2 + H2O→2 3-phospho-D-glycerate + 2 H+ (EC 4.1.1.39)
Ga0059261_2654 +0.1
3-phospho-D-glycerate→2-phospho-D-glycerate (EC 5.4.2.12)
Ga0059261_3169 +0.0
Phosphoglycerate kinase:
3-phospho-D-glycerate + ATP→3-phospho-D-glyceroyl phosphate + ADP (EC 2.7.2.3)
Ga0059261_0728 N.D.
Phosphopyruvate hydratase:
2-phospho-D-glycerate→H2O + phosphoenolpyruvate (EC 4.2.1.11)
Ga0059261_0014 +0.2
Pyruvate kinase (in reverse):
phosphoenolpyruvate + ADP + H+→pyruvate + ATP (EC 2.7.1.40)
Ga0059261_0685 +0.8
pyruvate + coenzyme A + NAD+→CO2 + acetyl-CoA + NADH (EC 1.2.1.104)
Ga0059261_0016 N.D.
Ga0059261_0017 N.D.
Ga0059261_3712 N.D.
Ga0059261_3715 N.D.
Acetyl-CoA C-acyltransferase:
2 acetyl-CoA→acetoacetyl-CoA + coenzyme A (EC 2.3.1.16; 2.3.1.9)
Ga0059261_0982 -0.2
Ga0059261_1483 -0.2
Ga0059261_2108 -0.1
Ga0059261_2160 +0.0
Ga0059261_2808 -0.2
Ga0059261_2835 +0.0
Ga0059261_2867 -0.1
Ga0059261_2877 -0.0
Ga0059261_2888 +0.1
Ga0059261_3284 -0.6
3-hydroxyacyl-CoA dehydrogenase (in reverse):
acetoacetyl-CoA + NADH + H+→(S)-3-hydroxybutanoyl-CoA + NAD+ (EC 1.1.1.35)
Ga0059261_0837 -0.2
Ga0059261_2534 -0.6
Ga0059261_2816 -0.1
Ga0059261_2827 -0.0
(S)-3-hydroxybutanoyl-CoA→crotonyl-CoA + H2O (EC 4.2.1.150)
Ga0059261_0515 +0.1
Ga0059261_0837 -0.2
Ga0059261_2159 -0.1
Ga0059261_2668 +0.0
Ga0059261_2803 -0.1
Ga0059261_2815 -0.1
Ga0059261_2818 -0.0
Ga0059261_2827 -0.0
Ga0059261_2840 -0.4
Ga0059261_2847 +0.0
Ga0059261_2858 +0.1
Ga0059261_2861 -0.1
Ga0059261_2887 -0.2
Ga0059261_2902 +0.0
Ga0059261_2908 -0.0
Ga0059261_3685 -2.3
Ga0059261_3986 +0.0
Ga0059261_4214 -0.6
Ga0059261_4222 +0.0
Trans-2-enoyl-CoA reductase (NAD(+)) (in reverse):
crotonyl-CoA + NADH + H+→butanoyl-CoA + NAD+ (EC 1.3.1.44)
Ga0059261_2800 -0.1
Ga0059261_2814 -0.1
Ga0059261_2828 +0.0
Ga0059261_2845 +0.0
Ga0059261_2856 +0.0
Ga0059261_2859 -0.2
Ga0059261_2907 -0.2
Ga0059261_3681 -0.1
Ga0059261_3793 +0.1
Butanal dehydrogenase (in reverse):
butanoyl-CoA + NAD(P)H + H+→1-butanal + coenzyme A + NAD(P)+ (EC 1.2.1.57)
No genes
1-butanal + NADH + H+→butan-1-ol + NAD+
No genes

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information