MetaCyc Pathway: pyruvate fermentation to hexanol (engineered) in Sphingomonas koreensis DSMZ 15582

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Reactions and Genes 5-Hydroxymethylfurfural 2.5 mM
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Pyruvate synthase:
pyruvate + coenzyme A + 2 an oxidized ferredoxin [iron-sulfur] cluster→acetyl-CoA + CO2 + H+ + 2 a reduced ferredoxin [iron-sulfur] cluster (EC 1.2.7.1)
No genes
Acetyl-CoA C-acyltransferase:
2 acetyl-CoA→acetoacetyl-CoA + coenzyme A (EC 2.3.1.16; 2.3.1.9)
Ga0059261_0982 -0.2
Ga0059261_1483 -0.2
Ga0059261_2108 -0.1
Ga0059261_2160 +0.0
Ga0059261_2808 -0.2
Ga0059261_2835 +0.0
Ga0059261_2867 -0.1
Ga0059261_2877 -0.0
Ga0059261_2888 +0.1
Ga0059261_3284 -0.6
3-hydroxyacyl-CoA dehydrogenase (in reverse):
acetoacetyl-CoA + NADH + H+→(S)-3-hydroxybutanoyl-CoA + NAD+ (EC 1.1.1.35)
Ga0059261_0837 -0.2
Ga0059261_2534 -0.6
Ga0059261_2816 -0.1
Ga0059261_2827 -0.0
(S)-3-hydroxybutanoyl-CoA→crotonyl-CoA + H2O (EC 4.2.1.150)
Ga0059261_0515 +0.1
Ga0059261_0837 -0.2
Ga0059261_2159 -0.1
Ga0059261_2668 +0.0
Ga0059261_2803 -0.1
Ga0059261_2815 -0.1
Ga0059261_2818 -0.0
Ga0059261_2827 -0.0
Ga0059261_2840 -0.4
Ga0059261_2847 +0.0
Ga0059261_2858 +0.1
Ga0059261_2861 -0.1
Ga0059261_2887 -0.2
Ga0059261_2902 +0.0
Ga0059261_2908 -0.0
Ga0059261_3685 -2.3
Ga0059261_3986 +0.0
Ga0059261_4214 -0.6
Ga0059261_4222 +0.0
Trans-2-enoyl-CoA reductase (NAD(+)) (in reverse):
crotonyl-CoA + NADH + H+→butanoyl-CoA + NAD+ (EC 1.3.1.44)
Ga0059261_2800 -0.1
Ga0059261_2814 -0.1
Ga0059261_2828 +0.0
Ga0059261_2845 +0.0
Ga0059261_2856 +0.0
Ga0059261_2859 -0.2
Ga0059261_2907 -0.2
Ga0059261_3681 -0.1
Ga0059261_3793 +0.1
Acetyl-CoA C-acyltransferase:
acetyl-CoA + butanoyl-CoA→3-oxohexanoyl-CoA + coenzyme A (EC 2.3.1.16; 2.3.1.9)
Ga0059261_0982 -0.2
Ga0059261_1483 -0.2
Ga0059261_2108 -0.1
Ga0059261_2160 +0.0
Ga0059261_2808 -0.2
Ga0059261_2835 +0.0
Ga0059261_2867 -0.1
Ga0059261_2877 -0.0
Ga0059261_2888 +0.1
Ga0059261_3284 -0.6
3-hydroxyacyl-CoA dehydrogenase (in reverse):
3-oxohexanoyl-CoA + NADH + H+→(S)-3-hydroxyhexanoyl-CoA + NAD+ (EC 1.1.1.35)
Ga0059261_0837 -0.2
Ga0059261_2534 -0.6
Ga0059261_2816 -0.1
Ga0059261_2827 -0.0
Long-chain-enoyl-CoA hydratase:
(S)-3-hydroxyhexanoyl-CoA→(2E)-hex-2-enoyl-CoA + H2O (EC 4.2.1.74)
Ga0059261_0515 +0.1
Ga0059261_0837 -0.2
Ga0059261_2159 -0.1
Ga0059261_2668 +0.0
Ga0059261_2803 -0.1
Ga0059261_2815 -0.1
Ga0059261_2818 -0.0
Ga0059261_2827 -0.0
Ga0059261_2840 -0.4
Ga0059261_2847 +0.0
Ga0059261_2858 +0.1
Ga0059261_2861 -0.1
Ga0059261_2887 -0.2
Ga0059261_2902 +0.0
Ga0059261_2908 -0.0
Ga0059261_3685 -2.3
Ga0059261_3986 +0.0
Ga0059261_4214 -0.6
Ga0059261_4222 +0.0
Trans-2-enoyl-CoA reductase (NAD(+)) (in reverse):
(2E)-hex-2-enoyl-CoA + NADH + H+→hexanoyl-CoA + NAD+ (EC 1.3.1.44)
No genes
hexanoyl-CoA + NAD(P)H + H+→1-hexanal + coenzyme A + NAD(P)+
No genes
1-hexanal + NADH + H+→hexan-1-ol + NAD+
No genes

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information