MetaCyc Pathway: L-valine degradation I in Azospirillum brasilense Sp245

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Reactions and Genes Paraquat dichloride 0.04 mg/ml
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Branched-chain-amino-acid transaminase:
L-valine + 2-oxoglutarate→3-methyl-2-oxobutanoate + L-glutamate (EC 2.6.1.42)
AZOBR_RS06555 -0.5
AZOBR_RS16425 -0.3
AZOBR_RS23800 -0.5
2-oxoisovalerate dehydrogenase (acylating):
3-methyl-2-oxobutanoate + coenzyme A + NAD+→isobutanoyl-CoA + CO2 + NADH (EC 1.2.1.25)
AZOBR_RS14390 N.D.
isobutanoyl-CoA + an oxidized electron-transfer flavoprotein + H+→methylacrylyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.5)
No genes
methylacrylyl-CoA + H2O→(S)-3-hydroxy-isobutanoyl-CoA (EC 4.2.1.150)
AZOBR_RS01260 -0.0
AZOBR_RS03680 +1.0
AZOBR_RS07775 -0.2
AZOBR_RS09775 N.D.
AZOBR_RS13325 +0.1
AZOBR_RS15730 +0.0
AZOBR_RS18155 +0.5
AZOBR_RS24105 -0.7
AZOBR_RS24715 +0.1
AZOBR_RS26270 -0.2
AZOBR_RS26485 +1.2
AZOBR_RS26830 +0.2
AZOBR_RS27635 -0.3
AZOBR_RS29210 +0.4
3-hydroxyisobutyryl-CoA hydrolase:
(S)-3-hydroxy-isobutanoyl-CoA + H2O→(S)-3-hydroxy-isobutanoate + coenzyme A + H+ (EC 3.1.2.4)
AZOBR_RS08460 +0.8
3-hydroxyisobutyrate dehydrogenase:
(S)-3-hydroxy-isobutanoate + NAD+→(S)-methylmalonate-semialdehyde + NADH + H+ (EC 1.1.1.31)
AZOBR_RS09555 -0.0
AZOBR_RS20225 -2.5
AZOBR_RS22360 -0.0
AZOBR_RS26815 -0.1
(S)-3-amino-2-methylpropionate transaminase (in reverse):
(S)-methylmalonate-semialdehyde + L-glutamate→(S)-3-amino-2-methylpropanoate + 2-oxoglutarate (EC 2.6.1.22)
AZOBR_RS19630 -0.5
Methylmalonate-semialdehyde dehydrogenase (acylating):
(S)-methylmalonate-semialdehyde + coenzyme A + NAD+ + H2O→propanoyl-CoA + hydrogen carbonate + NADH + H+ (EC 1.2.1.27)
AZOBR_RS22500 -0.1

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information