MetaCyc Pathway: superpathway of L-threonine biosynthesis in Enterobacter sp. TBS_079

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Reactions and Genes mutant library grown in Supernatant; Pseudomonas frederiksbergensis TBS_004 grown in LB
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Aspartate transaminase (in reverse):
oxaloacetate + L-glutamate→L-aspartate + 2-oxoglutarate (EC 2.6.1.1)
MPMX20_00609 +0.1
MPMX20_00612 -0.0
MPMX20_01049 +0.0
MPMX20_01059 +0.1
MPMX20_01157 -0.1
MPMX20_01586 -1.1
MPMX20_02048 +0.1
MPMX20_02230 +0.1
MPMX20_02425 -3.4
MPMX20_03220 -0.2
MPMX20_04048 +0.1
Aspartate kinase:
L-aspartate + ATP→L-aspartyl-4-phosphate + ADP (EC 2.7.2.4)
MPMX20_00240 +0.2
MPMX20_00710 +0.1
MPMX20_04530 -4.4
Aspartate-semialdehyde dehydrogenase (in reverse):
L-aspartyl-4-phosphate + NADPH + H+→L-aspartate 4-semialdehyde + NADP+ + phosphate (EC 1.2.1.11)
MPMX20_02127 -0.1
MPMX20_03254 +0.0
MPMX20_04315 N.D.
Homoserine dehydrogenase (in reverse):
L-aspartate 4-semialdehyde + NAD(P)H + H+→L-homoserine + NAD(P)+ (EC 1.1.1.3)
MPMX20_00710 +0.1
MPMX20_04530 -4.4
Homoserine kinase:
L-homoserine + ATP→O-phospho-L-homoserine + ADP + H+ (EC 2.7.1.39)
MPMX20_00711 -0.0
Threonine synthase:
O-phospho-L-homoserine + H2O→L-threonine + phosphate (EC 4.2.3.1)
MPMX20_00712 -0.1

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information