MetaCyc Pathway: superpathway of L-aspartate and L-asparagine biosynthesis in Enterobacter sp. TBS_079

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Reactions and Genes mutant library grown in Supernatant; Pseudomonas frederiksbergensis TBS_004 grown in LB
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Aspartate transaminase (in reverse):
oxaloacetate + L-glutamate→L-aspartate + 2-oxoglutarate (EC 2.6.1.1)
MPMX20_00609 +0.1
MPMX20_00612 -0.0
MPMX20_01049 +0.0
MPMX20_01059 +0.1
MPMX20_01157 -0.1
MPMX20_01586 -1.1
MPMX20_02048 +0.1
MPMX20_02230 +0.1
MPMX20_02425 -3.4
MPMX20_03220 -0.2
MPMX20_04048 +0.1
Aspartate ammonia-lyase (in reverse):
fumarate + ammonium→L-aspartate (EC 4.3.1.1)
MPMX20_00419 -2.4
Aspartate--ammonia ligase:
L-aspartate + ammonium + ATP→L-asparagine + AMP + diphosphate + H+ (EC 6.3.1.1)
MPMX20_01320 +0.1
MPMX20_04623 +0.1
Asparaginase:
L-asparagine + H2O→L-aspartate + ammonium (EC 3.5.1.1; 3.5.1.38; 3.5.5.4)
MPMX20_01849 -0.2
MPMX20_03348 +0.2

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information