MetaCyc Pathway: L-isoleucine biosynthesis IV in Dyella japonica UNC79MFTsu3.2

Reactions and Genes	L-Isoleucine (N)
Propionate--CoA ligase: propanoate + ATP + coenzyme A→propanoyl-CoA + AMP + diphosphate (EC 6.2.1.17)
ABZR86_RS08500	+0.0
ABZR86_RS15200	-0.0
3-methyl-2-oxobutanoate dehydrogenase (ferredoxin) (in reverse): propanoyl-CoA + CO2 + H+ + 2 a reduced ferredoxin [iron-sulfur] cluster→2-oxobutanoate + coenzyme A + 2 an oxidized ferredoxin [iron-sulfur] cluster (EC 1.2.7.7)
No genes
Acetolactate synthase: 2-oxobutanoate + H+ + pyruvate→(S)-2-aceto-2-hydroxybutanoate + CO2 (EC 2.2.1.6)
ABZR86_RS07005	+0.1
ABZR86_RS15930	N.D.
ABZR86_RS15935	-1.9
(S)-2-aceto-2-hydroxybutanoate + NAD(P)H + H+→(R)-2,3-dihydroxy-3-methylpentanoate + NAD(P)+ (EC 1.1.1.383)
No genes
Dihydroxy-acid dehydratase: (R)-2,3-dihydroxy-3-methylpentanoate→(3S)-3-methyl-2-oxopentanoate + H2O (EC 4.2.1.9)
ABZR86_RS04080	-0.0
ABZR86_RS15945	-1.3
Branched-chain-amino-acid transaminase (in reverse): (3S)-3-methyl-2-oxopentanoate + L-glutamate→L-isoleucine + 2-oxoglutarate (EC 2.6.1.42)
ABZR86_RS11580	-1.5
ABZR86_RS15965	-1.6

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information