MetaCyc Pathway: superpathway of L-isoleucine biosynthesis I in Dyella japonica UNC79MFTsu3.2

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Reactions and Genes L-Isoleucine (N)
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Aspartate transaminase (in reverse):
oxaloacetate + L-glutamate→L-aspartate + 2-oxoglutarate (EC 2.6.1.1)
ABZR86_RS01145 +0.1
ABZR86_RS02120 -0.1
ABZR86_RS03710 +0.1
ABZR86_RS04055 +0.1
ABZR86_RS08550 -0.1
ABZR86_RS11705 -0.0
ABZR86_RS12435 -3.5
ABZR86_RS14075 -0.0
ABZR86_RS17110 -0.1
ABZR86_RS20415 -0.2
Aspartate kinase:
L-aspartate + ATP→L-aspartyl-4-phosphate + ADP (EC 2.7.2.4)
ABZR86_RS15985 -0.5
ABZR86_RS19035 -0.3
ABZR86_RS19560 -2.6
Aspartate-semialdehyde dehydrogenase (in reverse):
L-aspartyl-4-phosphate + NADPH + H+→L-aspartate 4-semialdehyde + NADP+ + phosphate (EC 1.2.1.11)
ABZR86_RS08030 N.D.
Homoserine dehydrogenase (in reverse):
L-aspartate 4-semialdehyde + NAD(P)H + H+→L-homoserine + NAD(P)+ (EC 1.1.1.3)
ABZR86_RS15985 -0.5
ABZR86_RS19035 -0.3
Homoserine kinase:
L-homoserine + ATP→O-phospho-L-homoserine + ADP + H+ (EC 2.7.1.39)
ABZR86_RS15980 -1.6
Threonine synthase:
O-phospho-L-homoserine + H2O→L-threonine + phosphate (EC 4.2.3.1)
ABZR86_RS15975 -2.6
Threonine ammonia-lyase:
L-threonine→(Z)-2-aminobutenoate + H2O (EC 4.3.1.19)
ABZR86_RS14545 +0.5
ABZR86_RS15780 -0.2
ABZR86_RS15925 -0.1
1-aminocyclopropane-1-carboxylate deaminase:
(Z)-2-aminobutenoate→2-iminobutanoate (spontaneous) (EC 3.5.99.7; 4.3.1.19; 4.4.1.1; 4.4.1.11; 4.4.1.2)
ABZR86_RS14545 +0.5
ABZR86_RS15780 -0.2
ABZR86_RS15925 -0.1
ABZR86_RS18745 N.D.
2-iminobutanoate + H2O→2-oxobutanoate + ammonium (spontaneous) (EC 3.5.99.10; 3.5.99.7; 4.3.1.19; 4.4.1.1; 4.4.1.11; 4.4.1.2)
ABZR86_RS11845 -0.3
ABZR86_RS14545 +0.5
ABZR86_RS15780 -0.2
ABZR86_RS15925 -0.1
ABZR86_RS18745 N.D.
Acetolactate synthase:
2-oxobutanoate + H+ + pyruvate→(S)-2-aceto-2-hydroxybutanoate + CO2 (EC 2.2.1.6)
ABZR86_RS07005 +0.1
ABZR86_RS15930 N.D.
ABZR86_RS15935 -1.9
Ketol-acid reductoisomerase (in reverse):
(S)-2-aceto-2-hydroxybutanoate + NADPH + H+→(R)-2,3-dihydroxy-3-methylpentanoate + NADP+ (EC 1.1.1.86)
ABZR86_RS15940 -1.8
Dihydroxy-acid dehydratase:
(R)-2,3-dihydroxy-3-methylpentanoate→(3S)-3-methyl-2-oxopentanoate + H2O (EC 4.2.1.9)
ABZR86_RS04080 -0.0
ABZR86_RS15945 -1.3
Branched-chain-amino-acid transaminase (in reverse):
(3S)-3-methyl-2-oxopentanoate + L-glutamate→L-isoleucine + 2-oxoglutarate (EC 2.6.1.42)
ABZR86_RS11580 -1.5
ABZR86_RS15965 -1.6

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information