MetaCyc Pathway: 3-hydroxypropanoate/4-hydroxybutanate cycle in Sphingomonas koreensis DSMZ 15582

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Reactions and Genes casaminos (C)
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Carbonate dehydratase (in reverse):
CO2 + H2O→hydrogen carbonate + H+ (EC 4.2.1.1)
Ga0059261_0149 N.D.
Biotin carboxylase:
hydrogen carbonate + ATP + a [biotin carboxyl-carrier-protein dimer]-N6-biotinyl-L-lysine→a [carboxyl-carrier protein dimer]-N6-carboxybiotinyl-L-lysine + ADP + H+ + phosphate (EC 6.3.4.14)
Ga0059261_0291 N.D.
Ga0059261_0292 N.D.
a [carboxyl-carrier protein dimer]-N6-carboxybiotinyl-L-lysine + acetyl-CoA→malonyl-CoA + a [biotin carboxyl-carrier-protein dimer]-N6-biotinyl-L-lysine (EC 2.1.3.15)
Ga0059261_3596 N.D.
malonyl-CoA + NADPH + H+→3-oxopropanoate + coenzyme A + NADP+ (EC 1.2.1.75)
No genes
3-oxopropanoate + NADPH + H+→3-hydroxypropanoate + NADP+ (EC 1.1.1.298)
Ga0059261_1912 -0.2
3-hydroxypropanoate + ATP + coenzyme A→3-hydroxypropanoyl-CoA + AMP + diphosphate (EC 6.2.1.36)
No genes
3-hydroxypropanoyl-CoA→acryloyl-CoA + H2O (EC 4.2.1.116)
Ga0059261_3685 -0.3
acryloyl-CoA + NADPH + H+→propanoyl-CoA + NADP+ (EC 1.3.1.84)
No genes
Propionyl-CoA carboxylase:
hydrogen carbonate + propanoyl-CoA + ATP→(S)-methylmalonyl-CoA + ADP + H+ + phosphate (EC 6.4.1.3)
Ga0059261_0240 N.D.
Ga0059261_3596 N.D.
Ga0059261_3982 +0.9
Ga0059261_3993 +1.0
Ga0059261_4055 -1.0
Methylmalonyl-CoA epimerase (in reverse):
(S)-methylmalonyl-CoA→(R)-methylmalonyl-CoA (EC 5.1.99.1)
Ga0059261_3985 +0.5
Methylmalonyl-CoA mutase:
(R)-methylmalonyl-CoA→succinyl-CoA (EC 5.4.99.2)
Ga0059261_3988 +0.4
succinyl-CoA + NADPH + H+→succinate semialdehyde + coenzyme A + NADP+ (EC 1.2.1.76)
No genes
succinate semialdehyde + NADPH + H+→4-hydroxybutanoate + NADP+ (EC 1.1.1.co)
Ga0059261_0846 +0.0
4-hydroxybutanoate + ATP + coenzyme A→4-hydroxybutanoyl-CoA + AMP + diphosphate (EC 6.2.1.40)
No genes
4-hydroxybutanoyl-CoA→crotonyl-CoA + H2O (EC 4.2.1.120)
No genes
crotonyl-CoA + H2O→(S)-3-hydroxybutanoyl-CoA (EC 4.2.1.150)
Ga0059261_0515 +0.0
Ga0059261_0837 +0.0
Ga0059261_2159 -0.1
Ga0059261_2668 -0.1
Ga0059261_2803 -0.0
Ga0059261_2815 -0.3
Ga0059261_2818 +0.0
Ga0059261_2827 +0.1
Ga0059261_2840 -0.1
Ga0059261_2847 -0.1
Ga0059261_2858 +0.0
Ga0059261_2861 +0.1
Ga0059261_2887 -0.0
Ga0059261_2902 +0.1
Ga0059261_2908 +0.2
Ga0059261_3685 -0.3
Ga0059261_3986 +0.2
Ga0059261_4214 +0.1
Ga0059261_4222 +0.2
3-hydroxyacyl-CoA dehydrogenase:
(S)-3-hydroxybutanoyl-CoA + NAD+→acetoacetyl-CoA + NADH + H+ (EC 1.1.1.35)
Ga0059261_0837 +0.0
Ga0059261_2534 -0.4
Ga0059261_2816 -0.0
Ga0059261_2827 +0.1
Acetyl-CoA C-acyltransferase (in reverse):
acetoacetyl-CoA + coenzyme A→2 acetyl-CoA (EC 2.3.1.16; 2.3.1.9)
Ga0059261_0982 -0.1
Ga0059261_1483 -0.2
Ga0059261_2108 +0.0
Ga0059261_2160 +0.0
Ga0059261_2808 -0.0
Ga0059261_2835 -0.0
Ga0059261_2867 +0.0
Ga0059261_2877 -0.1
Ga0059261_2888 -0.0
Ga0059261_3284 -0.3

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information