MetaCyc Pathway: (8E,10E)-dodeca-8,10-dienol biosynthesis in Burkholderia phytofirmans PsJN

Reactions and Genes	L-Phenylalanine (N)
palmitoyl-CoA[in] + an oxidized electron-transfer flavoprotein[in] + H+[in]→(2E)-hexadec-2-enoyl-CoA[in] + a reduced electron-transfer flavoprotein[in] (EC 1.3.8.9)
No genes
(8E,10E)-dodeca-8,10-dienoate + 2 NADPH + 3 H+→(8E,10E)-dodeca-8,10-dienol + 2 NADP+ + H2O
No genes
Enoyl-CoA hydratase (in reverse): (2E)-hexadec-2-enoyl-CoA + H2O→(S)-3-hydroxyhexadecanoyl-CoA (EC 4.2.1.17)
BPHYT_RS02345	+0.2
BPHYT_RS03225	-0.1
BPHYT_RS03235	-0.6
BPHYT_RS03825	N.D.
BPHYT_RS06755	N.D.
BPHYT_RS06760	-0.8
BPHYT_RS08375	N.D.
BPHYT_RS08630	+0.1
BPHYT_RS09560	-0.3
BPHYT_RS10815	N.D.
BPHYT_RS11780	N.D.
BPHYT_RS13345	+0.5
BPHYT_RS13545	-1.0
BPHYT_RS14830	+0.6
BPHYT_RS17335	-2.5
BPHYT_RS17350	N.D.
BPHYT_RS20025	-1.0
BPHYT_RS25360	N.D.
BPHYT_RS25695	N.D.
BPHYT_RS27105	-0.1
BPHYT_RS27895	+0.5
BPHYT_RS28015	-0.1
BPHYT_RS28020	N.D.
BPHYT_RS28620	+0.5
BPHYT_RS35465	+0.3
Long-chain-3-hydroxyacyl-CoA dehydrogenase: (S)-3-hydroxyhexadecanoyl-CoA + NAD+→3-oxopalmitoyl-CoA + NADH + H+ (EC 1.1.1.211)
BPHYT_RS03225	-0.1
BPHYT_RS06760	-0.8
BPHYT_RS09560	-0.3
BPHYT_RS13545	-1.0
BPHYT_RS28020	N.D.
BPHYT_RS34155	+0.4
Acetyl-CoA C-myristoyltransferase (in reverse): 3-oxopalmitoyl-CoA + coenzyme A→myristoyl-CoA + acetyl-CoA (EC 2.3.1.155)
BPHYT_RS02230	-0.2
BPHYT_RS09565	+0.0
myristoyl-CoA + an oxidized electron-transfer flavoprotein + H+→(2E)-tetradec-2-enoyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.8)
No genes
Enoyl-CoA hydratase (in reverse): (2E)-tetradec-2-enoyl-CoA + H2O→(S)-3-hydroxytetradecanoyl-CoA (EC 4.2.1.17)
BPHYT_RS02345	+0.2
BPHYT_RS03225	-0.1
BPHYT_RS03235	-0.6
BPHYT_RS03825	N.D.
BPHYT_RS06755	N.D.
BPHYT_RS06760	-0.8
BPHYT_RS08375	N.D.
BPHYT_RS08630	+0.1
BPHYT_RS09560	-0.3
BPHYT_RS10815	N.D.
BPHYT_RS11780	N.D.
BPHYT_RS13345	+0.5
BPHYT_RS13545	-1.0
BPHYT_RS14830	+0.6
BPHYT_RS17335	-2.5
BPHYT_RS17350	N.D.
BPHYT_RS20025	-1.0
BPHYT_RS25360	N.D.
BPHYT_RS25695	N.D.
BPHYT_RS27105	-0.1
BPHYT_RS27895	+0.5
BPHYT_RS28015	-0.1
BPHYT_RS28020	N.D.
BPHYT_RS28620	+0.5
BPHYT_RS35465	+0.3
(S)-3-hydroxytetradecanoyl-CoA + NAD+→3-oxomyristoyl-CoA + NADH + H+
BPHYT_RS03225	-0.1
BPHYT_RS06760	-0.8
BPHYT_RS09560	-0.3
BPHYT_RS13545	-1.0
BPHYT_RS28020	N.D.
BPHYT_RS34155	+0.4
Acetyl-CoA C-acyltransferase (in reverse): 3-oxomyristoyl-CoA + coenzyme A→lauroyl-CoA + acetyl-CoA (EC 2.3.1.16)
BPHYT_RS02230	-0.2
BPHYT_RS03230	+0.2
BPHYT_RS08275	-0.0
BPHYT_RS09150	+0.3
BPHYT_RS09180	+0.1
BPHYT_RS09565	+0.0
BPHYT_RS17345	-0.1
BPHYT_RS21955	+0.5
BPHYT_RS29385	-0.6
lauroyl-CoA + 2 a ferrocytochrome b5 + dioxygen + 2 H+→(9E)-dodecenoyl-CoA + 2 a ferricytochrome b5 + 2 H2O
No genes
(9E)-dodecenoyl-CoA + 2 a ferrocytochrome b5 + dioxygen + 2 H+→(8E,10E)-dodeca-8,10-dienoyl-CoA + 2 a ferricytochrome b5 + 2 H2O
No genes

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information