MetaCyc Pathway: methyl ketone biosynthesis (engineered) in Burkholderia phytofirmans PsJN

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Reactions and Genes L-Phenylalanine (N)
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a fatty acid + ATP + coenzyme A→an acyl-CoA + AMP + diphosphate
No genes
Acyl-CoA oxidase:
a 2,3,4-saturated fatty acyl CoA + dioxygen→a (2E)-2-enoyl-CoA + hydrogen peroxide
(EC 1.3.3.6)
No genes
Enoyl-CoA hydratase (in reverse):
a (2E)-2-enoyl-CoA + H2O→a 2,3,4-saturated (3S)-3-hydroxyacyl-CoA
(EC 4.2.1.17)
BPHYT_RS02345 +0.2
BPHYT_RS03225 -0.1
BPHYT_RS03235 -0.6
BPHYT_RS03825 N.D.
BPHYT_RS06755 N.D.
BPHYT_RS06760 -0.8
BPHYT_RS08375 N.D.
BPHYT_RS08630 +0.1
BPHYT_RS09560 -0.3
BPHYT_RS10815 N.D.
BPHYT_RS11780 N.D.
BPHYT_RS13345 +0.5
BPHYT_RS13545 -1.0
BPHYT_RS14830 +0.6
BPHYT_RS17335 -2.5
BPHYT_RS17350 N.D.
BPHYT_RS20025 -1.0
BPHYT_RS25360 N.D.
BPHYT_RS25695 N.D.
BPHYT_RS27105 -0.1
BPHYT_RS27895 +0.5
BPHYT_RS28015 -0.1
BPHYT_RS28020 N.D.
BPHYT_RS28620 +0.5
BPHYT_RS35465 +0.3
3-hydroxyacyl-CoA dehydrogenase:
a 2,3,4-saturated (3S)-3-hydroxyacyl-CoA + NAD+→a 2,3,4-saturated 3-oxoacyl-CoA + NADH + H+
(EC 1.1.1.35)
BPHYT_RS03225 -0.1
BPHYT_RS06760 -0.8
BPHYT_RS09560 -0.3
BPHYT_RS13545 -1.0
BPHYT_RS34155 +0.4
a 2,3,4-saturated 3-oxoacyl-CoA + H2O→a 3-oxoacid + coenzyme A + H+
No genes
a 3-oxoacid + H+→a methyl ketone + CO2 (spontaneous)
No genes

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information