MetaCyc Pathway: 3-methylbutanol biosynthesis (engineered) in Burkholderia phytofirmans PsJN

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Reactions and Genes L-Isoleucine (N)
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2-isopropylmalate synthase:
3-methyl-2-oxobutanoate + acetyl-CoA + H2O→(2S)-2-isopropylmalate + coenzyme A + H+ (EC 2.3.3.13)
BPHYT_RS02790 -0.8
BPHYT_RS06570 +0.0
BPHYT_RS13280 -2.3
3-isopropylmalate dehydratase (in reverse):
(2S)-2-isopropylmalate→2-isopropylmaleate + H2O (EC 4.2.1.33)
BPHYT_RS13515 +0.2
BPHYT_RS13520 +0.2
BPHYT_RS23060 +0.4
BPHYT_RS33905 -1.2
BPHYT_RS33915 -5.9
3-isopropylmalate dehydratase (in reverse):
2-isopropylmaleate + H2O→(2R,3S)-3-isopropylmalate (EC 4.2.1.33)
BPHYT_RS13515 +0.2
BPHYT_RS13520 +0.2
BPHYT_RS23060 +0.4
BPHYT_RS33905 -1.2
BPHYT_RS33915 -5.9
3-isopropylmalate dehydrogenase:
(2R,3S)-3-isopropylmalate + NAD+→(2S)-2-isopropyl-3-oxosuccinate + NADH + H+ (EC 1.1.1.85)
BPHYT_RS33900 -3.9
3-isopropylmalate dehydrogenase:
(2S)-2-isopropyl-3-oxosuccinate + H+→4-methyl-2-oxopentanoate + CO2 (spontaneous) (EC 1.1.1.85)
BPHYT_RS33900 -3.9
Branched-chain-2-oxoacid decarboxylase:
4-methyl-2-oxopentanoate + H+→3-methylbutanal + CO2 (EC 4.1.1.72)
No genes
Alcohol dehydrogenase (in reverse):
3-methylbutanal + NADH + H+→3-methylbutanol + NAD+ (EC 1.1.1.1)
BPHYT_RS00510 +0.1
BPHYT_RS16150 -0.1
BPHYT_RS20205 -0.1
BPHYT_RS24470 -0.3
BPHYT_RS25430 N.D.
BPHYT_RS27390 +1.1
BPHYT_RS28135 -0.3
BPHYT_RS30075 -0.3
BPHYT_RS31305 +0.2
BPHYT_RS31860 N.D.

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information