MetaCyc Pathway: L-isoleucine biosynthesis II in Burkholderia phytofirmans PsJN

Reactions and Genes	L-Isoleucine (N)
pyruvate + acetyl-CoA + H2O→(R)-citramalate + coenzyme A + H+ (EC 2.3.3.21)
No genes
(R)-2-methylmalate dehydratase: (R)-citramalate→citraconate + H2O (EC 4.2.1.35)
BPHYT_RS13515	+0.2
BPHYT_RS13520	+0.2
BPHYT_RS23060	+0.4
BPHYT_RS33905	-1.2
BPHYT_RS33915	-5.9
citraconate + H2O→(2R,3S)-3-methylmalate
BPHYT_RS13515	+0.2
BPHYT_RS13520	+0.2
BPHYT_RS33905	-1.2
BPHYT_RS33915	-5.9
(2R,3S)-3-methylmalate + NAD+→2-oxobutanoate + CO2 + NADH
BPHYT_RS33900	-3.9
Acetolactate synthase: 2-oxobutanoate + pyruvate + H+→(S)-2-aceto-2-hydroxybutanoate + CO2 (EC 2.2.1.6)
BPHYT_RS06145	+0.2
BPHYT_RS06545	-5.3
BPHYT_RS06550	+0.0
BPHYT_RS10500	+0.8
BPHYT_RS19345	-0.1
BPHYT_RS31395	+0.6
BPHYT_RS34970	-0.2
(S)-2-aceto-2-hydroxybutanoate + NAD(P)H + H+→(R)-2,3-dihydroxy-3-methylpentanoate + NAD(P)+ (EC 1.1.1.383)
No genes
Dihydroxy-acid dehydratase: (R)-2,3-dihydroxy-3-methylpentanoate→(3S)-3-methyl-2-oxopentanoate + H2O (EC 4.2.1.9)
BPHYT_RS04815	-0.0
BPHYT_RS10100	-1.2
BPHYT_RS19730	-0.4
BPHYT_RS21270	-0.4
BPHYT_RS25650	-0.3
BPHYT_RS31695	-0.1
Branched-chain-amino-acid transaminase (in reverse): (3S)-3-methyl-2-oxopentanoate + L-glutamate→L-isoleucine + 2-oxoglutarate (EC 2.6.1.42)
BPHYT_RS16285	-2.4

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information