MetaCyc Pathway: L-isoleucine biosynthesis I (from threonine) in Burkholderia phytofirmans PsJN

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Reactions and Genes L-Isoleucine (N)
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Threonine ammonia-lyase:
L-threonine→(Z)-2-aminobutenoate + H2O (EC 4.3.1.19)
BPHYT_RS03145 N.D.
BPHYT_RS09280 +0.3
BPHYT_RS11065 N.D.
BPHYT_RS15425 -0.0
1-aminocyclopropane-1-carboxylate deaminase:
(Z)-2-aminobutenoate→2-iminobutanoate (spontaneous) (EC 3.5.99.7; 4.3.1.19; 4.4.1.1; 4.4.1.11; 4.4.1.2)
BPHYT_RS03145 N.D.
BPHYT_RS09280 +0.3
BPHYT_RS11065 N.D.
BPHYT_RS15425 -0.0
BPHYT_RS26815 -0.1
2-iminobutanoate + H2O→2-oxobutanoate + ammonium (spontaneous) (EC 3.5.99.10; 3.5.99.7; 4.3.1.19; 4.4.1.1; 4.4.1.11; 4.4.1.2)
BPHYT_RS03145 N.D.
BPHYT_RS09280 +0.3
BPHYT_RS11065 N.D.
BPHYT_RS15425 -0.0
BPHYT_RS26815 -0.1
BPHYT_RS29300 +0.5
Acetolactate synthase:
2-oxobutanoate + H+ + pyruvate→(S)-2-aceto-2-hydroxybutanoate + CO2 (EC 2.2.1.6)
BPHYT_RS06145 +0.2
BPHYT_RS06545 -5.3
BPHYT_RS06550 +0.0
BPHYT_RS10500 +0.8
BPHYT_RS19345 -0.1
BPHYT_RS31395 +0.6
BPHYT_RS34970 -0.2
Ketol-acid reductoisomerase (in reverse):
(S)-2-aceto-2-hydroxybutanoate + NADPH + H+→(R)-2,3-dihydroxy-3-methylpentanoate + NADP+ (EC 1.1.1.86)
BPHYT_RS06555 -5.7
Dihydroxy-acid dehydratase:
(R)-2,3-dihydroxy-3-methylpentanoate→(3S)-3-methyl-2-oxopentanoate + H2O (EC 4.2.1.9)
BPHYT_RS04815 -0.0
BPHYT_RS10100 -1.2
BPHYT_RS19730 -0.4
BPHYT_RS21270 -0.4
BPHYT_RS25650 -0.3
BPHYT_RS31695 -0.1
Branched-chain-amino-acid transaminase (in reverse):
(3S)-3-methyl-2-oxopentanoate + L-glutamate→L-isoleucine + 2-oxoglutarate (EC 2.6.1.42)
BPHYT_RS16285 -2.4

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information