MetaCyc Pathway: pyruvate fermentation to isobutanol (engineered) in Sphingomonas koreensis DSMZ 15582

Add experiment(s):


Reactions and Genes D-Xylose (C)
remove
Acetolactate synthase:
2 pyruvate + H+→(S)-2-acetolactate + CO2 (EC 2.2.1.6)
Ga0059261_0067 -5.3
Ga0059261_0068 -4.4
Ga0059261_0285 +0.3
Ga0059261_0603 +0.1
Ga0059261_1674 +0.0
Ketol-acid reductoisomerase (in reverse):
(S)-2-acetolactate + NADPH + H+→(2R)-2,3-dihydroxy-3-methylbutanoate + NADP+ (EC 1.1.1.86)
Ga0059261_0066 N.D.
Dihydroxy-acid dehydratase:
(2R)-2,3-dihydroxy-3-methylbutanoate→3-methyl-2-oxobutanoate + H2O (EC 4.2.1.9)
Ga0059261_2649 -4.2
Ga0059261_4216 -4.1
Branched-chain-2-oxoacid decarboxylase:
3-methyl-2-oxobutanoate + H+→isobutanal + CO2 (EC 4.1.1.72)
No genes
Alcohol dehydrogenase (in reverse):
isobutanal + NADH + H+→isobutanol + NAD+ (EC 1.1.1.1)
Ga0059261_0846 +0.1
Ga0059261_1819 -0.1
Ga0059261_2135 +0.0

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information