MetaCyc Pathway: biotin biosynthesis I in Burkholderia phytofirmans PsJN

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Reactions and Genes L-Glutamine (N)
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a malonyl-[acp] + S-adenosyl-L-methionine→a malonyl-[acp] methyl ester + S-adenosyl-L-homocysteine (EC 2.1.1.197)
No genes
Beta-ketoacyl-acyl-carrier-protein synthase II:
a malonyl-[acp] + a malonyl-[acp] methyl ester + H+→a 3-oxoglutaryl-[acp] methyl ester + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.179; 2.3.1.41)
BPHYT_RS14415 N.D.
BPHYT_RS15125 +0.1
BPHYT_RS15130 -0.1
BPHYT_RS15145 +0.5
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 3-oxoglutaryl-[acp] methyl ester + NADPH + H+→a (3R)-3-hydroxyglutaryl-[acp] methyl ester + NADP+ (EC 1.1.1.100)
BPHYT_RS01050 +0.1
BPHYT_RS04770 -0.2
BPHYT_RS05365 -0.3
BPHYT_RS05385 -0.0
BPHYT_RS06420 N.D.
BPHYT_RS07065 -0.1
BPHYT_RS08285 N.D.
BPHYT_RS08365 -0.1
BPHYT_RS08890 -0.2
BPHYT_RS10170 -0.1
BPHYT_RS10200 N.D.
BPHYT_RS10595 +0.2
BPHYT_RS10630 +0.3
BPHYT_RS10735 N.D.
BPHYT_RS11845 N.D.
BPHYT_RS13055 +0.6
BPHYT_RS14425 N.D.
BPHYT_RS15135 +0.2
BPHYT_RS15890 -0.9
BPHYT_RS16040 N.D.
BPHYT_RS16940 +0.3
BPHYT_RS20510 N.D.
BPHYT_RS20625 +0.1
BPHYT_RS20775 +0.2
BPHYT_RS21600 +0.2
BPHYT_RS22135 +0.5
BPHYT_RS25030 -0.2
BPHYT_RS25415 N.D.
BPHYT_RS25655 -0.3
BPHYT_RS26470 +0.6
BPHYT_RS28250 N.D.
BPHYT_RS30440 +0.3
BPHYT_RS30465 +0.5
BPHYT_RS30795 +0.1
BPHYT_RS31385 -0.3
BPHYT_RS31405 N.D.
BPHYT_RS31610 +0.1
BPHYT_RS31900 +0.3
BPHYT_RS33140 N.D.
BPHYT_RS33705 +0.3
BPHYT_RS34945 N.D.
BPHYT_RS34965 N.D.
BPHYT_RS35505 +0.2
BPHYT_RS35675 -0.3
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxyglutaryl-[acp] methyl ester→a (2E)-enoylglutaryl-[acp] methyl ester + H2O (EC 4.2.1.59)
BPHYT_RS12085 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E)-enoylglutaryl-[acp] methyl ester + NADH + H+→a glutaryl-[acp] methyl ester + NAD+ (EC 1.3.1.9)
BPHYT_RS01880 +0.1
BPHYT_RS12390 +0.1
BPHYT_RS20615 +0.3
BPHYT_RS30015 N.D.
BPHYT_RS33380 -0.1
Beta-ketoacyl-acyl-carrier-protein synthase II:
a glutaryl-[acp] methyl ester + a malonyl-[acp] + H+→a 3-oxopimeloyl-[acp] methyl ester + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.179; 2.3.1.41)
BPHYT_RS14415 N.D.
BPHYT_RS15125 +0.1
BPHYT_RS15130 -0.1
BPHYT_RS15145 +0.5
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 3-oxopimeloyl-[acp] methyl ester + NADPH + H+→a (3R)-3-hydroxypimeloyl-[acp] methyl ester + NADP+ (EC 1.1.1.100)
BPHYT_RS01050 +0.1
BPHYT_RS04770 -0.2
BPHYT_RS05365 -0.3
BPHYT_RS05385 -0.0
BPHYT_RS06420 N.D.
BPHYT_RS07065 -0.1
BPHYT_RS08285 N.D.
BPHYT_RS08365 -0.1
BPHYT_RS08890 -0.2
BPHYT_RS10170 -0.1
BPHYT_RS10200 N.D.
BPHYT_RS10595 +0.2
BPHYT_RS10630 +0.3
BPHYT_RS10735 N.D.
BPHYT_RS11845 N.D.
BPHYT_RS13055 +0.6
BPHYT_RS14425 N.D.
BPHYT_RS15135 +0.2
BPHYT_RS15890 -0.9
BPHYT_RS16040 N.D.
BPHYT_RS16940 +0.3
BPHYT_RS20510 N.D.
BPHYT_RS20625 +0.1
BPHYT_RS20775 +0.2
BPHYT_RS21600 +0.2
BPHYT_RS22135 +0.5
BPHYT_RS25030 -0.2
BPHYT_RS25415 N.D.
BPHYT_RS25655 -0.3
BPHYT_RS26470 +0.6
BPHYT_RS28250 N.D.
BPHYT_RS30440 +0.3
BPHYT_RS30465 +0.5
BPHYT_RS30795 +0.1
BPHYT_RS31385 -0.3
BPHYT_RS31405 N.D.
BPHYT_RS31610 +0.1
BPHYT_RS31900 +0.3
BPHYT_RS33140 N.D.
BPHYT_RS33705 +0.3
BPHYT_RS34945 N.D.
BPHYT_RS34965 N.D.
BPHYT_RS35505 +0.2
BPHYT_RS35675 -0.3
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxypimeloyl-[acp] methyl ester→a (2E)-enoylpimeloyl-[acp] methyl ester + H2O (EC 4.2.1.59)
BPHYT_RS12085 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E)-enoylpimeloyl-[acp] methyl ester + NADH + H+→a pimeloyl-[acp] methyl ester + NAD+ (EC 1.3.1.9)
BPHYT_RS01880 +0.1
BPHYT_RS12390 +0.1
BPHYT_RS20615 +0.3
BPHYT_RS30015 N.D.
BPHYT_RS33380 -0.1
a pimeloyl-[acp] methyl ester + H2O→a pimeloyl-[acp] + methanol + H+ (EC 3.1.1.85)
No genes
8-amino-7-oxononanoate synthase:
a pimeloyl-[acp] + L-alanine + H+→(8S)-8-amino-7-oxononanoate + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.47)
BPHYT_RS02160 -2.4
Adenosylmethionine--8-amino-7-oxononanoate transaminase:
(8S)-8-amino-7-oxononanoate + S-adenosyl-L-methionine→(7R,8S)-diaminononanoate + S-adenosyl-4-methylsulfanyl-2-oxobutanoate (EC 2.6.1.62)
BPHYT_RS02165 -3.6
BPHYT_RS27170 +0.0
BPHYT_RS30220 +0.2
Dethiobiotin synthase:
(7R,8S)-diaminononanoate + ATP + CO2→dethiobiotin + ADP + 3 H+ + phosphate (EC 6.3.3.3)
BPHYT_RS02155 -2.3
Biotin synthase:
dethiobiotin + H+ + 2 a reduced [2Fe-2S] ferredoxin + S-adenosyl-L-methionine + a sulfurated [sulfur carrier]→4,5-secobiotin + 5'-deoxyadenosine + L-methionine + 2 an oxidized [2Fe-2S] ferredoxin + an unsulfurated [sulfur carrier] (EC 2.8.1.6)
BPHYT_RS02150 -2.9
Biotin synthase:
4,5-secobiotin + S-adenosyl-L-methionine→biotin + 5'-deoxyadenosine + L-methionine + H+ (EC 2.8.1.6)
BPHYT_RS02150 -2.9

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information